This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Xiao-An Li, Jian-Ping Qing, Department of Gastroenterology, Chinese PLA, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China
Dian-Chun Fang, Pei-Ren Si, Ru-Gang Zhang, Liu-Qin Yang, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Supported by the Scientific Research Programs Foundation during the Tenth Five-Year Plan of PLA, No. 01MA172
Correspondence to: Dian-Chun Fang, Department of Gastroenterology,Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Received: August 24, 2002 Revised: September 20, 2002 Accepted: October 12, 2002 Published online: September 15, 2003
AIM
To observe the role of TRAIL in inducing apoptosis of SMMC-7721 cells.
METHODS
The survival fraction of SMMC-7721 cells was measured by MTT assay; Apoptosis rate was determined by TUNEL method and the ultramicrostructure of apoptosic cells induced by TRAIL was observed by electron-microscopy.
RESULTS
The role of TRAIL in survival fraction and apoptosis rate demonstrated a good relationship and the typic structure of apoptosic cells was foud in some cells treated by TRAIL.
CONCLUSION
TRAIL can induce apoptosis in SMMC-7721 cells.
Key Words: N/A
Citation: Li XA, Fang DC, Si PR, Zhang RG, Yang LQ, Qing JP. Role of TRAIL in inducing apoptosis of SMMC-7721 cells. Shijie Huaren Xiaohua Zazhi 2003; 11(9): 1369-1371
图1 TRAIL对肝癌SMMC-7721细胞凋亡率的影响.
A 未处理细胞, 胞膜微绒毛和伪足多见, 核大、核内有多个核仁; B 经TRAIL作用后的细胞线粒体空泡化; C 经TRAIL作用后的细胞, 胞膜微绒毛和伪足减少, 核浓缩、碎裂; D 经TRAIL作用后的细胞, 胞膜微绒毛和伪足减少, 凋亡小体形成.
Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA. Identification and characterization of a new member of the TNF family that induces apoptosis.Immunity. 1995;3:673-682.
[PubMed] [DOI]
Pitti RM, Marsters SA, Ruppert S, Donahue CJ, Moore A, Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family.J Biol Chem. 1996;271:12687-12690.
[PubMed] [DOI]
Evdokiou A, Bouralexis S, Atkins GJ, Chai F, Hay S, Clayer M, Findlay DM. Chemotherapeutic agents sensitize osteogenic sarcoma cells, but not normal human bone cells, to Apo2L/TRAIL-induced apoptosis.Int J Cancer. 2002;99:491-504.
[PubMed] [DOI]
Emery JG, McDonnell P, Burke MB, Deen KC, Lyn S, Silverman C, Dul E, Appelbaum ER, Eichman C, DiPrinzio R. Osteoprotegerin is a receptor for the cytotoxic ligand TRAIL.J Biol Chem. 1998;273:14363-14367.
[PubMed] [DOI]
Yamada H, Tada-Oikawa S, Uchida A, Kawanishi S. TRAIL causes cleavage of bid by caspase-8 and loss of mitochondrial membrane potential resulting in apoptosis in BJAB cells.Biochem Biophys Res Commun. 1999;265:130-133.
[PubMed] [DOI]
Rocklin OW, Guseva N, Tagiyev A, Knudson CM, Cohen MB. Bcl-2 oncoprotein protects the human prostatic carcinoma ell line PC3 from TRAIL-mediated apoptosis.Oncogene. 2001;20:2836-2843.
[PubMed] [DOI]
Thomas WD, Zhang XD, Franco AV, Nguyen T, Hersey P. TNF-related apoptosis-inducing ligand-induced apoptosis of melanoma is associated with changes in mitochondrial membrane potential and perinuclear clustering of mitochondria.J Immunol. 2000;165:5612-5620.
[PubMed] [DOI]
Pan G, O'Rourke K, Chinnaiyan AM, Gentz R, Ebner R, Ni J, Dixit VM. The receptor for the cytotoxic ligand TRAIL.Science. 1997;276:111-113.
[PubMed] [DOI]
Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL.Science. 1997;277:815-818.
[PubMed] [DOI]
Screaton GR, Mongkolsapaya J, Xu XN, Cowper AE, McMichael AJ, Bell JI. TRICK2, a new alternatively spliced receptor that transduces the cytotoxic signal from TRAIL.Curr Biol. 1997;7:693-696.
[PubMed] [DOI]
Walczak H, Degli-Esposti MA, Johnson RS, Smolak PJ, Waugh JY, Boiani N, Timour MS, Gerhart MJ, Schooley KA, Smith CA. TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL.EMBO J. 1997;16:5386-5397.
[PubMed] [DOI]
Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors.Science. 1997;277:818-821.
[PubMed] [DOI]
Degli-Esposti MA, Smolak PJ, Walczak H, Waugh J, Huang CP, DuBose RF, Goodwin RG, Smith CA. Cloning and characterization of TRAIL-R3, a novel member of the emerging TRAIL receptor family.J Exp Med. 1997;186:1165-1170.
[PubMed] [DOI]
MacFarlane M, Ahmad M, Srinivasula SM, Fernandes-Alnemri T, Cohen GM, Alnemri ES. Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL.J Biol Chem. 1997;272:25417-25420.
[PubMed] [DOI]
Marsters SA, Sheridan JP, Pitti RM, Huang A, Skubatch M, Baldwin D, Yuan J, Gurney A, Goddard AD, Godowski P. A novel receptor for Apo2L/TRAIL contains a truncated death domain.Curr Biol. 1997;7:1003-1006.
[PubMed] [DOI]
Degli-Esposti MA, Dougall WC, Smolak PJ, Waugh JY, Smith CA, Goodwin RG. The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain.Immunity. 1997;7:813-820.
[PubMed] [DOI]
Pan G, Ni J, Yu G, Wei YF, Dixit VM. TRUNDD, a new member of the TRAIL receptor family that antagonizes TRAIL signalling.FEBS Lett. 1998;424:41-45.
[PubMed] [DOI]
Sarker M, Ruiz-Ruiz C, Robledo G, Lopez-Rivas A. Stimulation of the mitogen-activated protein kinase pathway antagonizes TRAIL-induced apoptosis downstream of BID cleavage in human breast cancer MCF-7 cells.Oncogene. 2002;21:4323-4327.
[PubMed] [DOI]
Rohn TA, Wagenknecht B, Roth W, Naumann U, Gulbins E, Krammer PH, Walczak H, Weller M. CCNU-dependent potentiation of TRAIL/Apo2L-induced apoptosis in human glioma cells is p53-independent but may involve enhanced cytochrome c release.Oncogene. 2001;20:4128-4137.
[PubMed] [DOI]
Holler N, Zaru R, Micheau O, Thome M, Attinger A, Valitutti S, Bodmer JL, Schneider P, Seed B, Tschopp J. Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule.Nat Immunol. 2000;1:489-495.
[PubMed] [DOI]
Ozoren N, Kim K, Burns TF, Dicker DT, Moscioni AD, El-Deiry WS. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand.Cancer Res. 2000;60:6259-6265.
[PubMed] [DOI]