抑瘤基因NGX6对结肠癌血管形成的影响

图1 鸡胚绒毛尿囊膜血管(CAM)形成实验结果. A-B: 鸡胚孵育第7天; C: PBS对照组; D: HT-29组; E: pcDNA3.1(+)/HT-29组; F: pcDNA3.1(+)/NGX6/HT-29组.

图2 接种结肠癌细胞后裸鼠的体质量变化.

图3 第60天处理裸鼠时3组种植瘤比较. A: HT-29组; B: pcDNA3.1(+)/HT-29组; C: pcDNA3.1(+)/NGX6/HT-29组.

图4 3组裸鼠种植瘤HE染色病理结果(×200). A: HT-29组; B: pcDNA3.1(+)/HT-29组; C: pcDNA3.1(+)/NGX6/HT-29组.

图5 3组种植瘤微血管密度镜下观察结果(×200). A: pcDNA3.1(+)/NGX6/HT-29组; B: HT-29组; C: pcDNA3.1(+)/HT-29组.

图6 VEGF表达的RT-PCR鉴定结果. M: DL-2000; 1: H2O 阴性对照; 2: 未转染的HT-29细胞; 3: pcDNA3.1(+)/NGX6/HT-29细胞; 4: pcDNA3.1(+)/HT-29细胞; 5: 未转染的HT-29种植瘤; 6: pcDNA3.1(+)/NGX6/HT-29种植瘤; 7: pcDNA3.1(+)/HT-29种植瘤.

图7 3组裸鼠种植瘤癌栓组织病理图(HE×200). A: HT-29组; B: pcDNA3.1(+)/HT-29组; C: pcDNA3.1(+)/NGX6/HT-29组.

图8 3组裸鼠肺脏转移灶病理图(HE×200). A: HT-29组; B: pcDNA3.1(+)/HT-29组; C: pcDNA3.1(+)/NGX6/HT-29组.