shRNA干扰SMYD3对肝癌细胞c-Myc表达及凋亡的影响

图1 HepG2细胞中SMYD3、c-Myc的表达. A: 电泳图; 1: SMYD3、c-Myc; 2: β-actin; M: marker 2000 bp; B: 质粒转染24 h荧光倒置显微镜; C: 关闭可见光.

图2 质粒转染HepG2细胞后SMYD3和c-Myc的RT-PCR结果. A: 电泳图; B: SMYD3 mRNA表达量; C: c-Myc mRNA表达量. 1, 10: Pgenesil-1-s1转染24 h; 2, 11: Pgenesil-1-s2转染24 h; 3, 12: Pgenesil-1-hk转染24 h; 4, 13: Pgenesil-1-s1转染48 h; 5, 14: Pgenesil-1-s2转染48 h; 6, 15: Pgenesil-1-hk转染48 h; 7, 16: Pgenesil-1-s1转染72 h; 8, 17: Pgenesil-1-s2转染72 h; 9, 18: Pgenesil-1-hk转染72 h; M: marker 2000 bp; bP<0.01.

图3 shRNA干扰SMYD3后诱导HepG2细胞凋亡. A: 空白组; B: Pgenesil-1-hk转染; C: Pgenesil-1-s1转染; D: Pgenesil-1-s2转染.