抗体库优化策略对特异性抗肝癌单链抗体的人源化

图1 P1, P2, P3, P4, P5, P6, P7, P8和P9的PCR产物. M: marker; 1: P1; 2: P2; 3: P3; 4: P4; 5: P5; 6: P6; 7: P7; 8: P8; 9: P9

图2 P1-2、P3-4、P6-7和P8-9 PCR产物. M: marker; 1: P1-2; 2: P3-4; 3: P6-7; 4: P8-9

图3 P1-2-3-4、P6-7-8-9片段PCR产物. 1: P1-2-3-4; M: marker; 2: P6-7-8-9

图4 P1-9 PCR产物. M: marker; 1: P1-9

图5 重组子质粒DNA的PCR鉴定图. M: marker; 1: P1-2; 2: P1-4; 3: P1-6; 4: P3-9; 5: P1-8; 6: P1-9

图6 硫氰酸盐洗脱法比较亲本克隆DM和阳性克隆HDM1, HDM2, HDM3相对亲和力