Effect of cisapride on intestinal bacterial and endotoxin translocation in cirrhosis

Table 1 Characteristics of control and cirrhotic rats

	Control rats	Cirrhotic rats
Number of animal	25	25
IBO(%)	0	20/25(80%)b
Total jejunal bacteria contents (CFU/mL)	0.54 ± 0.18	1.59 ± 0.48b
Bacterial translocation(%)	0	12/25(48%)b
Endotoxin level(pg/mL)	0.11 ± 0.058	0.648 ± 0.134b
Intestinal transit (geometric center ratio)	0.49 ± 0.08	0.31 ± 0.06a
Intestinal permeability (%urinary excretionof 99mTc-DTPA)	1.62 ± 0.8	16.1 ± 7.6b

^bP < 0.01 vs control rats;

^aP < 0.05 vs control rats.

Table 2 Characteristics of Cirrhotic rats with and without BT

	Cirrhotic rats with BT	Cirrhotic rats without BT
Number of animal	12	13
IBO(%)	100%	63%a
Total jejunal bacteria contents (CFU/mL)	2.61 ± 0.56	0.65 ± 0.12b
Endotoxin level(pg/mL)	0.873 ± 0.137	0.440 ± 0.108b
Intestinal transit (geometric center ratio)	0.24 ± 0.06	0.38 ± 0.11a
Intestinal permeability (%urinary excretion of 99mTc-DTPA)	22.2 ± 7.8	10.5 ± 2.9b

^bP < 0.01 vs cirrhotic rats with BT;

^aP < 0.05 vs cirrhotic rats with BT.

Table 3 Bacterial species cultured from mesenteric lymph nodes, liver, spleen, peripheral blood and overgrowth in the jejunum

No.	Mesentericlymph nodes	Liver	Spleen	Blood	Intestinal bacterial overgrowth
1	E.coli	E.coli	E.coli	-	E.coli
2	E.coli	E.coli	E.coli	E.coli	E.coli
3	E.coli	E.coli	E.coli	E.coli	E.coli
					E.faecalis
4	E.coli	E.coli	E.coli	E.coli	E.coli
5	-	E.coli	-	-	E.coli
6	E.coli	E.coli	-	-	E.coli
	Ps. aeruginosa				Ps. aeruginosa
7	E.coli	-	E.coli	-	E.coli
8	P klebsiella	-	-	-	P klebsiella
	E.coli				E.coli
9	E.coli	E.coli	-	-	E.coli
	P. mirabilis				P. mirabilis
10	-	E.coli	E.coli	-	E.coli
11	P enterococus	-	SP	-	P enterococus
	E.coli		enterococus		E.coli
12	E.coli	-	-	E.coli	E.coli

Abbreviations: E. coli, Escherichia coli; P. mirabilis, Proteus mirabilis; Ps. Aeruginosa, Pseudomonas aeruginosa, P klebsiella: Pneumonia Klebsiella.

Table 4 Effect of cisapride on BT, IBO, serum endotoxin level, intestinal transit and intestinal permeability of cirrhotic rats

	Placebo	Cisapride
Number of animal	20	20
Bacterial translocation (%)	11/20(55%)	2/20(10%)b
IBO(%)	16/20(80%)	3/20(15%)b
Total jejunal bacteria contents (CFU/mL)	1.60 ± 0.42	0.60 ± 0.58a
Endotoxin level(pg/mL)	0.721 ± 0.123	0.148 ± 0.079b
Intestinal transit (geometric center ratio)	0.33 ± 0.08	0.68 ± 0.16b
Intestinal permeability (%urinary excretion of 99mTc-DTPA)	17.2 ± 5.98	12.2 ± 5.28a

^bP < 0.01 vs placebo;

^aP < 0.05 vs placebo.