Inhibition of conjugated linoleic acid on mouse forestomach neoplasia induced by benzo (a) pyrene and chemopreventive mechanisms

Table 1 The diets given after 2 wk of giving BP

Group	diets
Salad oil Control (A)	Standard diet + salad oil: fat (salad oil) concentration was 20%
BP Control (B)	Standard diet + salad oil: fat (salad oil) concentration was 20%
BP + 75% c9,t11-CLA (C)	Standard diet + salad oil + 75% c9,t11-CLA: Fat (salad oil) concentration was 20%, CLA 0.8%
BP + 98% c9,t11-CLA (D)	Standard diet + salad oil + 98% c9,t11-CLA: Fat (salad oil) concentration was 20%, CLA 0.5%
BP + 98% t10,c12-CLA (E)	Standard diet + salad oil + 98% t10,c12-CLA: Fat (salad oil) concentration was 20%, CLA 0.5%

Table 2 Inhibition of BP-induced forestomach neoplasia in female KunMing mice by CLA

Group	No. of mice (No. of tumor-bearing mice)/treatment	Total number of tumors	Tumor incidence (%)	Tumors/tumor-bearing moused	Diameter/tumor (CM)
A	14 (0)	0	0	0	0
B	12 (12)	31	100	2.58 ± 0.90	0.216 ± 0.088
C	12 (9)	23	75.0a	2.56 ± 0.73	0.157 ± 0.047e
D	13 (9)	22	69.2bc	2.44 ± 0.53	0.127 ± 0.038e
E	13 (7)	21	53.8bc	3.00 ± 0.58	0.128 ± 0.077e

^aP > 0.05 compared to the group B;

^bP < 0.05 compared to the group B;

^cP > 0.05 compared to the group C;

^dP > 0.05,

^eP < 0.05 compared to the group B

Table 3 The effects of CLA on mouse plasma lipid peroxidation

Group	MDA in plasma (nmol/L)
A	9.995 ± 1.634
B	9.937 ± 1.854
C	17.668 ± 4.610ab
D	14.005 ± 4.116a
E	13.303 ± 3.593a

^aP < 0.05 compared to the group A, B;

^bP < 0.05 compared to the group D, E.

Table 4 The effects of CLA on apoptosis in mice stomach (10³ cells)

Group	Apoptotic cell numbers
A	42.63 ± 6.02
B	30.88 ± 3.72
C	144.00 ± 20.31a
D	153.75 ± 23.25a
E	157.25 ± 15.95a

^aP < 0.01 compared to the group A, B.