Copyright
©The Author(s) 2003.
World J Gastroenterol. Jan 15, 2003; 9(1): 169-173
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.169
Published online Jan 15, 2003. doi: 10.3748/wjg.v9.i1.169
Table 1 Antagonism of different of concentrations of monoclonal antibody (MAb) to methionine enkephalin (MENK) at 1 × 10-6 mol·L-1 on the activity of PKA in cytosol and membrane of NS-1 cells. n = 3 from 3 independent experiments. bP < 0.05 and cP < 0.01 vs control, eP < 0.05 and fP < 0.01 vs group 2
MENK+MAb | Activity of PKA in cytosol/pmol·mg-1·min-1 | Activity of PKA in membrane/pmol·mg-1·min-1 |
Control | 21.10 ± 1.09 | 6.81 ± 1.63 |
MENK | 24.69 ± 0.49c | 10.42 ± 0.71b |
MENK+10-11 mol·L-1 MAb | 29.98 ± 0.59cf | 13.73 ± 1.84ce |
MENK+10-10 mol·L-1 MAb | 20.03 ± 1.47 | 7.59 ± 0.35e |
MENK+10-9 mol·L-1 MAb | 12.99 ± 0.95cf | 7.11 ± 2.04e |
Table 2 Antagonism of different of concentrations of monoclonal antibody (MAb) to methionine enkephalin (MENK) at 1 × 10-6 mol·L-1 on the intracellular activity of PKC in NS-1 cells. n = 3 from 3 independent experiments. bP < 0.05 vs control, eP < 0.05 and fP < 0.05 vs group 2
MENK+MAb | Activity of PKC (pmol·mg-1·min-1) |
Control | 3.06 ± 0.19 |
MENK | 2.26 ± 0.03b |
MENK+10-11 mol·L-1 MAb | 2.92 ± 0.02e |
MENK+10-10 mol·L-1 MAb | 2.80 ± 0.4e |
MENK+10-9 mol·L-1 MAb | 3.13 ± 0.45ff |
- Citation: Liu XH, Huang DA, Yang FY, Hao YS, Du GG, Li PF, Li G. A new cytokine: the possible effect pathway of methionine enkephalin. World J Gastroenterol 2003; 9(1): 169-173
- URL: https://www.wjgnet.com/1007-9327/full/v9/i1/169.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i1.169