Alterations of p53 and PCNA in cancer and adjacent tissues from concurrent carcinomas of the esophagus and gastric cardia in the same patient in Linzhou, a high incidence area for esophageal cancer in northern China

Table 1 Expression of P53 protein in 25 CC patients

Number	Sex/age	P53 immunostaining
		SCC		GCA
		(+/-)a	Immunostaining pattern	(+/-a)	Immunostaining pattern
001	M/63	+a	Diffuse	-a	-
002	M/60	-	-	+	Focus & Scattered
003	M/55	+	Diffuse	-	-
004	M/71	+	Diffuse & Scattered	+	Scattered
005	F/56	+	Scattered & Diffuse	-	-
006	M/59	-	-	-	-
007	F/67	-	-	-	-
008	M/62	+	Diffuse	+	Diffuse
009	F/61	+	Diffuse	-	-
010	F/64	+	Scattered	-	-
011	M/65	+	Diffuse	-	Diffuse
012	M/68	+	Diffuse	-	Diffuse
013	M/57	-	-	-	-
014	M/51	+	Diffuse	-	-
015	M/50	-	-	-	-
016	M/67	+	Scattered	-	Diffuse
017	M/70	+	Focus	-	-
018	M/67	+	Scattered	-	Scattered
019	F/74	-	-	+	Diffuse
020	M/49	-	-	+	Diffuse
021	M/36	-	-	-	-
022	M/70	+	Diffuse	-	-
023	F/74	-	-	-	-
024	F/56	-	-	-	-
025	F/38	+	Scattered	+	Diffuse & Scattered

^a+: positive immunostaining of P53, -: negative immunostaining of P53.

Table 2 Expression of PCNA protein in 25 CC patients

Number	Sex/age	PCNA immunostaining
		SCC		GCA
		(+/-)a	Immunostaining pattern	(+/-a)	Immunostaining pattern
001	M/63	+a	Diffuse	+a	Diffuse
002	M/60	+	Scattered	+	Focus & Scattered
003	M/55	+	Diffuse	+	Diffuse
004	M/71	+	Scattered & Focus	+	Diffuse
005	F/56	+	Scattered & Diffuse	+	Diffuse
006	M/59	+	Scattered & Focus	+	Diffuse
007	F/67	+	Diffuse	+	Focus
008	M/62	+	Diffuse	+	Diffuse
009	F/61	+	Diffuse	+	Diffuse
010	F/64	+	Scattered	+	Diffuse
011	M/65	+	Focus	+	Diffuse
012	M/68	+	Diffuse	+	Diffuse
013	M/57	-	-	-	-
014	M/51	+	Diffuse	-	-
015	M/50	+	Scattered	+	Diffuse
016	M/67	+	Scattered	+	Diffuse
017	M/70	+	Focus	+	Scattered & Focus
018	M/67	+	Diffuse	+	Scattered
019	F/74	+	Diffuse	+	Diffuse
020	M/49	+	Scattered & Focus	+	Diffuse
021	M/36	+	Diffuse	+	Diffuse
022	M/70	-	-	-	-
023	F/74	+	Diffuse	+	Diffuse
024	F/56	+	Scattered & Focus	+	Diffuse
025	F/38	+	Scattered	+	Diffuse

^a+: positive immunostaining of PCNA, +: negative immunostaining of PCNA.

Table 3 Expression of P53 & PCNA in precancerous lesions of adjacent cancerous tissues

	Number of samples	P53		PCNA
		+	%	-	%
Esophagus
NOR	11	3	27	6	55
BCH	10	5	50	7	70
DYS	8	4	50	6	75
CIS	7	2	29	5	71
SCC	14	10	71	13	93
Gastric cardia
NOR	9	4	44	6	67
DYS	11	3	27	7	64
CIS	9	2	22	6	67
CGA	14	5	36	12	86