Salvia miltiorrhiza monomer IH764-3 induces hepatic stellate cell apoptosis via caspase-3 activation

Table 1 The effect of IH764-3 on ³H-TdR incorporation (^-x±s)

Dosage (μg·mL-1)	Cpm	Inhibitory rate (%)
0	19749 ± 7222
1	018339 ± 5344	7.1
2	014147 ± 1850b	28.4
3	09125 ± 916b	53.8
4	05313 ± 426b	73.1

^bP < 0.01 vs control

Table 2 Apoptotic rates of HSCs exposed IH764-3 with different doses by Annexin-V/PI (%)

Time doses	0 μg·mL-1	10 μg·mL-1	20 μg·mL-1	30 μg·mL-1	40 μg·mL-1
12 hr	0.3	2.2	3.7	6.7	15.1
48 hr	2.3	6.7	9.3	15.1	19.7

Table 3 Percentage of apoptotic HSCs exposed to IH764-3 (30 μg·mL^-1) at different time points by Annexin-V/PI (%)

Time	0 hr	12 hr	24 hr	48 hr	72 hr
Apoptosis rate	0.3	6.7	10.3	15.1	19.6

Table 4 Fluorescence index of caspase-3 protein in HSCs (^-x±s)

Dosage (μg·mL-1)	Caspase-3	P value
0	175.6 ± 22.5
10	215.1 ± 26.9	< 0.01
20	231.6 ± 28.6	< 0.01
30	304.7 ± 30.7	< 0.01
40	338.9 ± 32.1	< 0.01