Expression and function of classical protein kinase C isoenzymes in gastric cancer cell line and its drug-resistant sublines

Table 1 Adriamycin accumulation and retention in cells by flow cytometric analysis (^-x±s fluorescence intensity)

Adriamycin	SGC7901	SGC7901/VCR in differentresistant drug dose (VCR, mg·L-1)
		0.3	0.7	1.0
Accumulation	5.21 ± 2.56	0.85 ± 0.29b	0.81 ± 0.32b	0.80 ± 0.33b
Retention	2.51 ± 1.23	0.47 ± 0.14b	0.44 ± 0.15b	0.41 ± 0.11b

^bP < 0.01, vs SGC7901.

Table 2 Effects of adriamycin accumulation in SGC7901/VCR cells by anti-PKC isoenzymes Ab (^-x±s fluorescence intensity)

Group	ρ (anti-PKC isoenzymes Ab)/(μg·L-1)
	0	25	250	500
Anti-PKC-α Ab	1.14 ± 0.36	1.09 ± 0.32	2.49 ± 0.84b	2.71 ± 0.94b
Anti-PKC-β IAb	1.14 ± 0.36	1.13 ± 0.38	1.14 ± 0.39	1.14 ± 0.39
Anti-PKC-α IIAb	1.14 ± 0.36	1.14 ± 0.38	1.14 ± 0.40	1.14 ± 0.39
Anti-PKC-γ Ab	1.14 ± 0.36	1.14 ± 0.39	1.14 ± 0.40	1.14 ± 0.39

^bP < 0.01, vs 0 μg·L^-1 anti-PKC isoenzymes Ab