Screening and identification of proteins mediating senna induced gastrointestinal motility enhancement in mouse colon

Table 1 Carthartic effect of SE in different administration routes

	Groups	Dose/g	n	Administration routes	Diarrhea (in 6 h)
I	Normal saline	0.3	6	ig	-
II	SE	0.3	6	ig	+(6)b
III	Normal saline	0.3	6	im	-
IV	SE	0.3	6	im	-
VC	SE	0.3	6	sc	-

^bP < 0.01, vs all other groups.

Table 2 Effect of SE on gastrointestinal propellant rate in mice (^-x±s)

	Group	Dose/g	Administration routes	n	Propellant rate/%
I	Normal saline	0.3	ig	10	45.8 ± 14.6
II	SE	0.3	ig	10	65.1 ± 7.5b
III	SE	0.3	im	10	48.3 ± 12.4

^bP < 0.01, vs I, III groups.

Table 3 Identification of the differential proteins

No.	Molecular mass/ku	Isoelectric point/pI	No.	Molecular mass/ ku	Isoelectric Point/pI
1	15.0	3.9	11	18.0	5.9
2	17.5	5.2	12	33.0	4.3
3	19.0	4.7	13	34.0	4.5
4	26.0	4.6	14	35.0	4.6
5	18.0	8.8	15	24.0	5.1
6	17.5	8.6	16	26.0	5.2
7	16.5	8.5	17	24.5	5.7
8	17.0	8.0	18	25.0	6.1
9	20.0	7.0	19	25.0	6.5
10	16.0	6.6	20	52.0	7.0