Activity of boanmycin against colorectal cancer

Table 1 Inhibitory effect of BAM on the growth of human colorectal xenografts in nude mice*

Drug	Tumor	No. of mice	Dose (mg/kg)	Tumor weight (g) x-± s	Tumor growth inhibition (%)
		6		1.300 ± 0.620
	HT-29	6	10	0.138 ± 0.064	89b
		6	15	0.098 ± 0.074	92b
BAM
	Hce-8693	5		1.501 ± 1.445
		5	10	0.154 ± 0.125	90b
		5	15	0.122 ± 0.154	92b

*Treatment was started on day 3 and day 14 after HT-29 and Hce-8693 tumor transplantation, ip., twice a week, with a total of 10 injections.

^bP < 0.01, vs control group.

Table 2 Inhibitory effect of BAM, MMC and 5-Fu on the growth and tumor cell mitosis of human colon cancer HT-29 xenografts in nude mice*

Drug	Dose** (mg/kg)	No. of mice	Tumor weight (g) x-± s	Inhibitory rate (%)	Necrotic ratio of umors△(%)	Mitotic figures△△
						x-± s	(%)
Control		5	0.804 ± 0.173		35b	104 ± 12	100
BAM	10	5	0.148 ± 0.059	82	67	41 ± 4	39b
MMC	1	5	0.376 ± 0.174	53a	43b	107 ± 12	103
5-FU	27	5	0.707 ± 0.168	12b	35b	130 ± 17	130

*Treatment was started on day 9 after tumor transplantation, ip., twice a week, with a total of 10 injections;

^**Drugs were administered at equitoxic doses (1/9 LD50);

^△Ratio of necrotic areas in whole section of the tumor.

^△△Number of mitotic figures in 10 optical fields of the section of tumor.

^aP < 0.05, vs BAM group;

^bP < 0.01, vs BAM group.

Table 3 Inhibitory effect of BAM, MMC and 5-FU on the growth of CT-26 tumor at the cecum and s.c site in mice*

Exp.	Drug	Dose** (mg/kg)	No. of mice	s.c. tumor		Cecal tumor
				Tumor weight (g) x-± s	Inhibitory rate (%)	Tumor weight (g) x-± s	Inhibitory rate (%)
I	Control		9	0.854 ± 0.151		0.557 ± 0.112
	BAM	10	9	0.101 ± 0.054	88b	0.005 ± 0.010	99b
	MMC	1	8	0.311 ± 0.105	64	0.159 ± 0.043	71
	5-FU	27	8	0.459 ± 0.118	46	0.310 ± 0.148	44
II	Control		6	0.900 ± 0.396		0.740 ± 0.446
	BAM	10	6	0.105 ± 0.088	88b	0.011 ± 0.019	99b
	MMC	1	5	0.674 ± 0.270	25	0.159 ± 0.510	78
	5-FU	27	6	0.657 ± 0.322	27	0.434 ± 0.255	41

*Treatment was started on next day after tumor cell injection, im., qod., with a total of 10 injections.

^**Drugs were administered at equitoxic doses (1/9 LD50).

^bP < 0.01, vs any other group.

Table 4 Bone marrow nucleated cells in mice bearing CT-26 treated by BAM, 5-FU and MMC

Exp.	Drug	Dose** (mg/kg)	No. of mice	Nucleated cells (106/femur)
				x-± s	%
I	Control		6	13 ± 3	100
	BAM	10	6	13 ± 3	100
	MMC	1	5	7 ± 2	54b
	5-FU	27	6	7 ± 2	54b
II	Control	9		12 ± 3	100
	BAM	10	9	12 ± 2	100
	MMC	1	8	3 ± 1	25b
	5-FU	27	8	4 ± 1	33b

*Drugs were administered at equitoxic doses (1/9 LD50).

^bP < 0.01, vs control group.