Influencing factors of rat small intestinal epithelial cell cultivation and effects of radiation on cell proliferation

Table 1 Effect of density on ³H-TdR incorporation in culture cell (x^-± s)

Density (× 104/well)	n	Min-1	Density (× 104/well)	n	Min-1
1.25	18	3547 ± 681	11.25	20	23648 ± 1398
2.50	20	9941 ± 413	12.50	18	20593 ± 2245
5.00	18	17931 ± 2051	13.75	20	19812 ± 2310
7.50	18	19825 ± 2135	15.00	18	17638 ± 959
10.00	24	23789 ± 2536	17.50	22	14874 ± 881

Table 2 Effect of culture time on IEC-6 cell proliferation (x^-± s)

Culture time (h)	n	Min-1	Culture time (h)	n	Min-1
6	24	1846 ± 146	48	24	21258 ± 1240
12	26	4038 ± 363	72	24	25727 ± 2006
24	24	6367 ± 588	96	24	24355 ± 2079

Table 3 Effect of ³H-TdR dosage on IEC-6 cell proliferation (x^-± s)

3H-TdR dosage (kBq/well)	n	Min-1	3H-TdR dosage (kBq/well)	n	Min-1
0.00	20	139 ± 29	27.75	21	27555 ± 1637
2.31	22	1333 ± 118	37.00	21	37235 ± 1485
4.62		10136 ± 1083	46.25	22	41874 ± 1213
9.25	24	16880 ± 1447	55.05	24	48072 ± 1676
18.50	24	24890 ± 1623	74.00	20	42430 ± 1735

Table 4 Effect of pH of culture medium on ³H-TdR incorporation (x^-± s)

pH	n	Min-1	pH	n	Min-1
6.0	20	4528 ± 660	7.6	28	12897 ± 1301
6.6	24	18771 ± 920	8.0	20	1305 ± 146
7.26	24	22510 ± 1448	8.8	20	636 ± 102

Table 5 Effect of insulin and concentration of fetal bovine serum on IEC-6 cell proliferation (x^-± s)

Fetal bovine serum (%)	n	Min-1
		Insulin group	Control group
0.0	18	476 ± 22	510 ± 101
2.5	20	13111 ± 978	1901 ± 580
5.0	22	14756 ± 1094	9097 ± 1069
7.5	22	20262 ± 2012	14569 ± 1136
10.0	24	23666 ± 1114	18775 ± 1361
15.0	20	22743 ± 1728	17645 ± 1289
20.0	18	22590 ± 1603	16965 ± 1147

Table 6 Effect of ionizing radiation dosage on ³H-TdR incorporation in IEC-6 cell (x^-± s)

Dosage (Gy)	Min-1	(%)	Dosage (Gy)	Min-1	(%)
0	13427 ± 803	100.0	16	3698 ± 371	15.2
4	7736 ± 765	31.8	18	3381 ± 235	13.9
6	7249 ± 472	29.8	20	3041 ± 327	12.5
8	5863 ± 594	24.1	22	2725 ± 348	11.2
10	4865 ± 586	20.0	24	2481 ± 263	10.2
14	4136 ± 424	17.0	26	2019 ± 154	8.3
		r = -0.970