Islet separation and islet cell culture in vitro from human embryo pancreas

Table 1 Contents of insulin and C-peptide in human embryo islet cell culture solution

Islet cells (culture days)	Contents of insulin (IU/L)	Contents of C-peptide (mg/L)
3	47.30	3.05
5	64.75	6.05
7	72.30	6.20
9	72.70	> 6.00
11	68.75	5.15
13	72.20	> 6.00

*Insulin antibody is negative in all tubes.

Table 2 Changes of blood sugar, insulin, C-peptide after experiment al treatment in normal and diabetes rabbits (x^-± s)

Experimental rabbits	Blood sugar (g/L)	Contents of insulin (IU/L)	Contents of C-peptide (mg/L)
Normal controls	1.0524 ± 0.0510	6.27 ± 4.6	13 .23 ± 4.77
Diabetes rabbits	4.7979 ± 0.9233	2.65 ± 1.4	Not detected
Experimentally	3.3193 ± 0.4110a	13.88 ± 1.5 a	Not detected
treated rabbits
P	< 0.01	< 0.05

^aAfter transplanted with rabbit islet cells, diabetes rabbits showed good spirit, less drinking and urine excretion.