Apoptosis of neoplasm cell lines induced by hepatic peptides extracted from sucking porcine hepatocytes

Table 1 Effect of pHGF and its fractions on normal hepatocytes an d hepatoma cells (x^-± s, n = 8)

	pHGF (OD, mg/L)			Fractions (OD, 20 mg/L)						Control
	500	1000	2000	1	2	3	4	5	6
7402 cells	0.422 ± 0.11b	0.410 ± 0.09b	0.401 ± 0.07b	0.545 ± 0.03	0.601 ± 0.11	0.427 ± 0.067b	0.411 ± 0.09b	0.580 ± 0.08	0.649 ± 0.21	631 ± 0.12
Primary hepatocytes	0.390 ± 0.07b	0.398 ± 0.09b	0.388 ± 0.06b	0.293 ± 0.06b	0.308 ± 0.06b	0.223 ± 0.07	0.236 ± 0.06	0.280 ± 0.10b	0.219 ± 0.11	0.218 ± 0.07

^bP < 0.01, vs control.

Table 2 Effects of S4 on 8 neoplasm cell lines at different time points

Cells	IC50 (mg/L)
	24 h	48 h	72 h
BEL-7402	8.2	5.0	3.9
SMMC-7721		8.4	4.2
QGY-7703		24.2	9.5
Hepe	15.2	8.9	6.5
SGC-7901	30.2	19.7	14.3
GLC-821	2.4	9.9	5.4
CNE-2			8.9
HCT-8			9.9

IC₅₀: The half inhibitory concentration.

Table 3 Apoptosis of 8 neoplasm cell line induced by S4 (5 mg/L, 48 h)

	Control	S4 Treatment
BEL-7402	+	++
SMMC-7721	+	++
QGY-7703	±	++
Hepe	+	++
HCT-8	±	±
GLC-82	±	+
SGC-7901	±	+
CNE-2	++	+

Table 4 Effects of S4 on the 4 oncogene expressions of 8 neoplasm cell lines (S4, 5 μg/well, 48 h)

	P53		Fas		Bcl-2		c-myc
	C	T	C	T	C	T	C	T
BEL-7402	+	++	+	++	+	±	+	+
SMMC-7721	+	++	+++	+++	+	±	++	++
QGY-7703	±	+	+	+++	±	±	+	++
Hepe	+	++	++	++	++	±	++	+
HCT-8	+	+	++	++	±	±	++	+
GLC-82	++	+	+++	+++	+	±	++	+
SGC-7901	+	+	±	+	+	±	++	+
CNE-2	+	++	+++	++	±	+	++	+

C: Control group; T: Test group.

Table 5 Inhibition of S4 on hepatoma cells in nude mice (x^-± s, n = 6)

	Dose (mg/kg)	Do (mm3)	Dn (mm3)	Dn-Do (mm3)	Inhibitoryrate (%)
High dose of S4	2.0	2.19 ± 0.24	2.81 ± 0.49	0.61 ± 0.38	59b
Low dose of S4	1.0	2.00 ± 0.12	2.55 ± 0.22	0.56 ± 0.26	63b
Doxorubicin	1.0	2.26 ± 0.12	2.16 ± 0.32	0.10 ± 0.29	107b
Control	0.0	2.17 ± 0.23	3.67 ± 0.52	1.50 ± 0.55

Do: Dimension of tumors at onset; Dn: Dimension of tumors at necropsy.

^bP < 0.01, vs control.

Table 6 Apoptosis of hepatoma cells in nude mice induced by S4 (LI, x^-± s, n = 6)

	Dose (mg/kg)	LI (%)
High dose of S4	2.0	18.70 ± 4.92b
Low dose of S4	1.0	13.22 ± 1.74b
Doxorubincin	1.0	14.18 ± 2.46b
Control	0.0	8.40 ± 2.81

^bP < 0.01, vs control. LI: Lable index.