Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma

Table 1 The pathological changes of rat livers during different stages of liver cancer induced with 2-FAA

Groups	n	Pathological feature (HE staining)
		Degeneration	Precancerosis	Cancerization
Control	6	0	0	0
2nd week	6	6	0	0
4th week	6	3	3	0
6th week	6	0	5	1
8th week	6	0	4	2
10th week	6	0	1	5
12th week	5a	0	0	5

^aOne rat died during the experiment.

Table 2 Changes of total RNA concentrations and GGT specific activities at different stages of rat hepatomas

Groups	n	Total RNA (μg/mg tissue)	GGT specific activity
			Liver GGT (U/g)	Serum GGT (U/L)
Control	6	3.67 ± 0.78	1.06 ± 0.24	6.0 ± 4.8
2nd week	6	6.69 ± 6.08	4.77 ± 4.41a	20.2 ± 17.8a
4th week	6	4.81 ± 4.27	9.30 ± 1.81b	31.2 ± 28.1b
6th week	6	5.38 ± 4.84	5.95 ± 1.23b	37.8 ± 32.1b
8th week	6	9.23 ± 6.48a	10.73 ± 4.93b	53.5 ± 43.6b
10th week	6	8.37 ± 6.51a	16.03 ± 5.22b	40.6 ± 23.6b
12th week	5	8.16 ± 8.87a	15.71 ± 3.31b	38.6 ± 8.8b

^aP < 0.05,

^bP < 0.01, compared with the control group.

Table 3 The changes in specific activities of different GGT forms at different stages of rat hepatomas

Groups	n	Hepatic homogenate GGT (U/g)
		S (Soluble-type)	T-S (Membrane-type)	S/T-GGT
Control	6	0.74 ± 0.14	0.32 ± 0.14	0.71 ± 0.08
2nd week	6	3.35 ± 2.83a	1.42 ± 0.23	0.72 ± 0.14
4th week	6	7.07 ± 0.92b	2.29 ± 1.28b	0.76 ± 0.10
6th week	6	3.50 ± 0.70b	2.45 ± 1.25b	0.60 ± 0.15
8th week	6	8.68 ± 5.24b	2.39 ± 1.52b	0.74 ± 0.18
10th week	6	12.21 ± 5.49b	3.82 ± 1.32b	0.73 ± 0.13
12th week	5	9.56 ± 2.27b	6.15 ± 4.36b	0.64 ± 0.24

^aP < 0.05,

^bP < 0.01, compared with the control group.