Codon 249 mutations of p53 gene in non-neoplastic liver tissues

Table 1 Primers for PCR and AS-PCR

Code	Oligonucleotide sequence	Position
S1	5′-GGCGACAGAGCGAGATTCCA	13890-13909
A1	5′-GATTCTCTTCCTCTGTGCGC	14534-14515
S2	5′-TGGGCGGCATGAACCGGAGT	14055-14074
S3	5′-TGGGCGGCATGAACCGGAGG	14055-14074
A2	5′-GGGTCAGCGGCAAGCAGAGG	14175-14156

Table 2 Codon 249 AGT transversion detected in cancerous and non-neoplastic liver tissues detected by PCR/SSCP

Tissue types	Case number	SSCP positive cases	Positive rates (%)
Chronic hepatitis	10	0	0
Cirrhosis	5	0	0
Pericancerous tissues	20	0	0
Cancerous tissues	20	9	45

Table 3 Detection of codon 249 AGT transversion of p53 gene in cancerous and pericancerous tissues

Tissues types	Cases	Detective rates of codon 249 mutation (%)
Chronic hepatitis	10	70
Cirrhosis	5	100
Pericancerous tissues	20	70
Cancerous tissues	20	65