Effect of cytokines on liver necrosis

Table 1 The changes of liver function after cytokines injection in mice (^-x±s)

	n	TBIL(μmol/L)	ALT(IU/L)	AST(IU/L)
Control	6	7.28 ± 4.82	46 ± 4	187 ± 28
rTNFα	3	8.70 ± 5.34	50 ± 11	89 ± 27
rIL-1	3	7.23 ± 0.87	40 ± 5	76 ± 10
rIL-6	3	11.77 ± 3.51	31 ± 1	62 ± 2
rIFNγ	3	9.30 ± 2.7	31 ± 5	89 ± 13

F = 0.8 between groups, P > 0.05

Table 2 The combined effect of rTNFα with rIL-1 or rIL-6 or rIFNγ on liver function of normal mice (^-x±s)

	n	TBIL(μmol/L)	ALT(IU/L)	AST(IU/L)
Control	6	7.28 ± 4.82	46 ± 4	187 ± 28
rTNFα	3	8.70 ± 5.34	50 ± 11	89 ± 27
rTNFα + rIFNγ	3	7.38 ± 6.01	32 ± 3	100 ± 14
rTNFα + rIL-1	3	8.59 ± 5.9	42 ± 11	128 ± 19
rTNFα + rIL-6	3	10.85 ± 3.25	36 ± 5	115 ± 12

F = 0.8, between groups, P > 0.05.

Table 3 The effect of D-GAL with cytokines on TBIL and histological changes of liver

Group	n	TBIL(μmol/L)	Histological changes (n)
			±	+	++
Control	6	7.28 ± 4.82	6
D-GAL	6	26.67 ± 11.14		6
D + rIL-1	6	29.69 ± 9.6		6
D + rIL-6	6	45.55 ± 12.9a		1	5
D + rIFNγ	6	41.94 ± 14.9a		2	4
D + rTNFα	6	46.19 ± 10.62a		2	4

^aP < 0.05, vs group D-GAL.

Table 4 The effects of DGal and rTNFα with rIL-1, rIL-6, rIFNγ separately on TBIL and hepatic cell of mice

Group	n	BIL(μmol/L) (-x±s)	Histological changes
			-or ±	+	++	+++	++++
Control	6	7.28 ± 4.8	6
D + rTNFα	6	46.19 ± 10.6		2	4
D + rTNFα + rIL-1	6	66.27 ± 18.7a				3	3a
D + rTNFα + rIL-6	6	69.28 +18.9a				2	4a
D + rTNFα + rIFNγ	6	36.75 ± 14.5		2	4

^aP < 0.05, vs group of D + rTNFα.