Expression of alpha fetoprotein messenger RNA in BEL-7404 human hepatoma cells and effect of L-4-oxalysine on the expression

Table 1 Probes used in this experiments

Plasmids	Carrier	Gene contented	Inserted	Restrictive enzyme	Resistance
pHAF-2	pBR322	AFP	900 bp	Pst I + Hap II	Tetracycline
phalb-7	pBR322	Albumin	727 bp	Pst I + Hind III	Tetracycline

Table 2 Influence of L-4-oxalysine (OXL) on AFP mRNA expression in BEL-7404 human hepatoma cells

Treatment	Concentration (mg/L)	Positive intensities of AFP mRNA (A470 ± S)	Inhibition (%)
Control		0.67 ± 0.08
OXL	25	0.44 ± 0.06a	34.3
OXL	50	0.20 ± 0.05b	70.1

BEL-7404 human hepatoma cells (5 × 10⁶ cells/L) were cultivated for 72 h in absence or presence of various concentrations of OXL. The results were expressed as absorbance at 470 nm (A470). The percentage of inhibition was calculated in the cultures in absence of OXL.

^aP < 0.05;

^bP < 0.01.