Bicuculline ameliorates metabolic dysfunction-associated steatotic liver disease by inhibiting the nuclear factor-kappa B pathway and reducing lipid accumulation

Table 1 Primer sequences of zebrafish for RT-qPCR

Primer	Primer sequence (5’-3’)	Primer sequence (5’-3’)
β-actin	ATGGATGAGGAAATCGCTGCC	CTCCCTGATGTCTGGGTCGTC
Srebp1	CATCCACATGGCTCTGAGTG	CTCATCCACAAAGAAGCGGT
Fasn	GCACCGGTACTAAGGTTGGA	ACACAACCGACCATCTGTCA
Scd1	ACGCTCCTCAGATACGCACT	AGTCGTAGGGAAACGTGTGG
Hmgcr	CCTGTTAGCCGTCAGTGGA	TCTTTGACCACTCGTGCCG
APOA1	GCACTAAGCTGACCGAGCGT	GGAGGTCCTGGGTGTGTGGA
PPARγ	TGCTGGACTACCAGAACTGTGACA	TGCTGGCTGAGAACACTTCTGAG
IL-6	CCTCAAACCTTCAGACCGCT	GAACAGGATCGAGTGGACCG
TNF-α	GCTTATGAGCCATGCAGTGA	TGCCCAGTCTGTCTCCTTCT

Table 2 Primer sequences of mouse for RT-qPCR

Primer	Primer sequence (5’-3’)	Primer sequence (5’-3’)
GAPDH	CGACTTCAACAGCAACTCCCACTCTTCC	TGGGTGGTCCAGGGTTTCTTACTCCTT
Srebf1	AGCCATCGACTACATCCG	TCCATAGACACATCTGTGCCTC
Fasn	TGGTGGTGTGGACATGGTCACAGA	CCGAAGCTGGGGGTCCATTGTGTG
Scd1	ACACCATGGCGTTCCAGAAT	AGCTTCTCGGCTTTCAGGTC
Dgat1	TCGTGGTATCCTGAATTGGTG	AGGTTCTCTAAAAATAACCTTGCATT
Dgat2	GCTGGTGCCCTACTCCAAG	CCAGCTTGGGGACAGTGA
IL-1β	GAAATGCCACCTTTTGACAGTG	TGGATGCTCTCATCAGGACAG
COX-2	TTCCAATCCATGTCAAAACCGT	AGTCCGGGTACAGTCACACTT
TNF-α	AGGAGGAATTTGGCCAGGTG	GCTCACGAGGAGGCTAATCC