Lactococcus garvieae aggravates cholestatic liver disease by increasing intestinal permeability and enhancing bile acid reabsorption

doi:10.3748/wjg.v31.i10.101014

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Mar 14, 2025 (publication date) through Feb 28, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2025; 31(10): 101014
Published online Mar 14, 2025. doi: 10.3748/wjg.v31.i10.101014

Table 1 The primer sequences used in real-time-qPCR

Gene	Forward sequence	Reverse sequence
Mouse ZO-1	GGGCCATCTCAACTCCTGTA	AGAAGGGCTGACGGGTAAAT
Mouse occludin	ACTATGCGGAAAGAGTTGACAG	GTCATCCACACTCAAGGTCAG
Mouse TNFα	GAAGTTCCCAAATGGCCTCC	GTGAGGGTCTGGGCCATAGA
Mouse IL-1β	CTTTGAAGTTGACGGACCC	TGAGTGATACTGCCTGCCTG
Mouse IL-6	CTAGGTTTGCCGAGTAGATCTC	GACAAAGCCAGAGTCCTTCAGAGA
Mouse α-SMA	AGCCATCTTTCATTGGGATGG	CCCCTGACAGGACGTTGTTA
Mouse COL1A1	CCCTGGTCCCTCTGGAAATG	GGACCTTTGCCCCCTTCTTT
Mouse COL1A2	TGCGTACCTGGATGAGGAGA	GAGCAGCCATCGACTAGGAC
Mouse COL3A1	CCCACAGCCTTCTACACCTG	CCAGGGTCACCATTTCTCCC
Mouse COL4A2	CGGCGTAATCTCAAAAGGCG	GGCCTCTGCTTCCTTTCTGT
Mouse TGFβ	GCTGAACCAAGGAGACGGAAT	GCTGATCCCGTTGATTTCCA
Mouse CCL2	AGCTGTAGTTTTTGTCACCAAGC	GTGCTGAAGACCTTAGGGCA
Mouse CCL5	TGCTGCTTTGCCTACCTCTC	TCCTTCGAGTGACAAACACGA
Mouse CCL20	CGACTGTTGCCTCTCGTACA	GCTTCATCGGCCATCTGTCT
Mouse P16	GCTCAACTACGGTGCAGATTC	GCACGATGTCTTGATGTCCC
Mouse ASBT	GCGACATGGACCTCAGTGTTA	GTTCCCGAGTCAACCCACAT
Mouse OATP	CCTGGAGCAGCAATATGGAAA	CCAAGGCATACTGGAGGCAA
Mouse NTCP	TACCTCCTCCCTGATGCCTTTC	TGCGTCTGCAGCTTGGATTTA
Mouse BSEP	CAATGTTCAGTTCCTCCGTTCA	TTTGGTGTTGTCCCCGTGCTTG
Mouse MDR1	CCCCCGAGATTGACAGCTAC	ACTCCACTAAATTGCACATTTCCTTC
Mouse CYP7A1	TACTAGATAGCATCATCAAGGAGGCTC	CCATCCTCAAGGTGCAGAGTG
Mouse CYP8B1	GGTACGCTTCCTCTATCGCC	GAGGGATGGCGTCTTATGGG
Mouse CYP27A1	GAAGCCATCACCTATATC	ATAGACTGAGTTCTGGAA
Mouse GAPDH	TTGATGGCAACAATCTCCAC	CGTCCCGTAGACAAAATGGT
Human ZO-1	TGCTGAGTCCTTTGGTGATG	AATTTGGATCTCCGGGAAGAC
Human occludin	GTTGCGGCGAGCGGATTG	TGGACTTTCAAGAGGCCTGG
Human TNFα	GCTGCACTTTGGAGTGATCG	GCTTGAGGGTTTGCTACAACA
Human CCL2	TCAAACTGAAGCTCGCACTC	TGGGGCATTGATTGCATCTGG
Human CCL5	CACCCTGCTGCTTTGCCTA	CATCCTTGACCTGTGGACGACT
Human GAPDH	GCTCCTCCTGTTCGACAGTCA	ACCTTCCCCATGGTGTCTGA

ZO-1: Zonula occludens-1; TNFα: Tumor necrosis factor alpha; IL: Interleukin; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; CYP7A1: Cytochrome P450 family 7 subfamily A member 1; CYP27A1: Cytochrome P450 family 27 subfamily A member 1.

Citation: Liu M, Ji YL, Hu YJ, Su YX, Yang J, Wang XY, Chu HY, Zhang X, Dong SJ, Yang H, Liu YH, Zhou SM, Guo LP, Ran Y, Li YN, Zhao JW, Zhang ZG, Piao MY, Zhou L. Lactococcus garvieae aggravates cholestatic liver disease by increasing intestinal permeability and enhancing bile acid reabsorption. World J Gastroenterol 2025; 31(10): 101014
URL: https://www.wjgnet.com/1007-9327/full/v31/i10/101014.htm
DOI: https://dx.doi.org/10.3748/wjg.v31.i10.101014