Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model

doi:10.3748/wjg.v30.i5.471

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Feb 7, 2024 (publication date) through Jun 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 7, 2024; 30(5): 471-484
Published online Feb 7, 2024. doi: 10.3748/wjg.v30.i5.471

Table 1 The primers used in the present study

Target	Oligonucleotide sequence (5’-3’)
Target	Forward primer	Reverse primer
let-7a-5p	GGAGGTAGTTCGTTGTGTGGT
U6	ATGGGTCGAAGTCGTAGCC	TTCTCGGCGTCTTCTTTCTCG
Gfp	TGCTTCAGCCGCTACCC	AGTTCACCTTGATGCCGTTC
β-actin	AACTCCATCATGAAGTGTGA	CTGCGGCTTCTATTGGGGAC
Tnfa	CTTGTTGCCTCCTCTTTTGCTTA	GACTTCAGCACTCAAGACATCC
Il6	TCACAGAAGGAGTGGCTAAGGACC	ACGCACTAGGTTTGCCGAGTAGAT
Acta2	TTCATCGGGATGGAGTCTGCTGG	TCGGTCGGCAATGCCAGGGT
Tgfb	CCACCTGCAAGACCATCGAC	CTGGCGAGCCTTAGTTTGGAC
Ccl₂	TTAAAAACCTGGATCGGAACCAA	GCATTAGCTTCAGATTTACGGGT

Citation: Hua H, Zhao QQ, Kalagbor MN, Yu GZ, Liu M, Bian ZR, Zhang BB, Yu Q, Xu YH, Tang RX, Zheng KY, Yan C. Recombinant adeno-associated virus 8-mediated inhibition of microRNA let-7a ameliorates sclerosing cholangitis in a clinically relevant mouse model. World J Gastroenterol 2024; 30(5): 471-484
URL: https://www.wjgnet.com/1007-9327/full/v30/i5/471.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i5.471