Transfection of 12/15-lipoxygenase effectively alleviates inflammatory responses during experimental acute pancreatitis

Table 1 Primers used in real-time transcription polymerase chain reaction (real-time PCR)

Primer		Sequence	Nucleotide length
IL-1β	Forward	5′-CACCTTCTTTTCCTTCATCTTTG-3′	23
Reverse	5′-GTCGTTGCTTGTCTCTCCTTGTA-3′	23
IL-6	Forward	5′-TGATGGATGCTTCCAAACTGGA-3′	22
Reverse	5′-GAGCATTGGAAGTTGGGGTA-3′	20
TNF-α	Forward	5′-ACTGAACTTCGGGGTGATTGG-3′	21
Reverse	5′-GCTTGGTGGTTTGCTACGAC-3′	20
MCP-1	Forward	5′-TCTCACTTGGTTCTGGTCCAGT-3′	22
Reverse	5′-GGCCTGTTGTTCACAGTTGCT-3′	21
Alox15	Forward	5′-GTCTACTCCACCACCTATTTTC-3′	22
Reverse	5′-CCTGTGCTCATTGCCTTGTC-3′	20
GAPDH	Forward	5′-TGACTTCAACAGCGACACCCA-3′	21
	Reverse	5′-GCTGTTGGGCTGTAGGGA-3′	18

TNF-α: Tumor necrosis factor-α; MCP-1: Monocyte chemoattractant protein-1; IL: Interleukin.