Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway

doi:10.3748/wjg.v30.i20.2709

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May 28, 2024 (publication date) through Jan 13, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2024; 30(20): 2709-2725
Published online May 28, 2024. doi: 10.3748/wjg.v30.i20.2709

Table 1 Primers used for quantitative real-time PCR

Gene	Species	Forward primer (5′-3′)	Reverse primer (5′-3′)
SIRT1	Human	CAAGAGGCCGCGGAGAGATG	CGGCCCATTGTCTCCTTCCC
Nrf2	Human	TTCAGCCAGCCCAGCACATC	ACGGGAATGTCTGCGCCAAA
HO-1	Human	CAAGCAGCTCTACCGCTCCC	GGGTGTTGAGTGGGGGCTTC
GAPDH	Human	CAATGCCTCCTGCACCACCA	GATGTTCTGGAGAGCCCCGC
SIRT1	Rat	CCCGGACAGTTCCAGCCATC	GGCACCTAGGACACCGAGGA
Nrf2	Rat	CCGATCTGCTCGGCGAAGTT	ACAAAGTGGTGCTGGCCTCC
HO-1	Rat	AGAGGCCTGCTAGCCTGGTT	TGTGAGGACCCATCGCAGGA
GAPDH	Rat	TGTGGATGGCCCCTCTGGAA	TGACCTTGCCCACAGCCTTG

Citation: Chen KD, Wang KL, Chen C, Zhu YJ, Tang WW, Wang YJ, Chen ZP, He LH, Chen YG, Zhang W. Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1/nuclear factor-erythroid-2-related factor 2/heme oxygenase-1 signaling pathway. World J Gastroenterol 2024; 30(20): 2709-2725
URL: https://www.wjgnet.com/1007-9327/full/v30/i20/2709.htm
DOI: https://dx.doi.org/10.3748/wjg.v30.i20.2709