HepG2.2.15-derived exosomes facilitate the activation and fibrosis of hepatic stellate cells

Table 1 Differentially expressed miRNA

miRNA_ID	log2FC	Q value	Sequence
hsa-let-7a-5p	-2.73154	0.00204	TGAGGTAGTAGGTTGTATAGTT
hsa-let-7b-5p	-4.69951	2.33E-07	TGAGGTAGTAGGTTGTGTGGTT
hsa-let-7i-5p	-2.38322	0.00143	TGAGGTAGTAGTTTGTGCTGTT
hsa-miR-1-3p	4.033866	0.047597	TGGAATGTAAAGAAGTATGTAT
hsa-miR-100-5p	-3.94205	1.30E-07	AACCCGTAGATCCGAACTTGTG
hsa-miR-10a-5p	-3.59889	2.26E-05	TACCCTGTAGATCCGAATTTGTG
hsa-miR-10b-5p	2.666194	0.000232	TACCCTGTAGAACCGAATTTGTG
hsa-miR-1269a	6.487066	0.002002	CTGGACTGAGCCGTGCTACTGG
hsa-miR-146a-5p	5.734318	6.49E-10	TGAGAACTGAATTCCATGGGTT
hsa-miR-146b-5p	4.145809	8.31E-05	TGAGAACTGAATTCCATAGGCTG
hsa-miR-148a-3p	1.906654	0.019359	TCAGTGCACTACAGAACTTTGT
hsa-miR-148a-5p	6.152472	0.006559	AAAGTTCTGAGACACTCCGACT
hsa-miR-181a-2-3p	-4.83032	0.047597	ACCACTGACCGTTGACTGTACC
hsa-miR-185-5p	2.986783	0.03238	TGGAGAGAAAGGCAGTTCCTGA
hsa-miR-192-5p	2.596432	0.041346	CTGACCTATGAATTGACAGCC
hsa-miR-193b-5p	-5.73663	0.011076	CGGGGTTTTGAGGGCGAGATGA
hsa-miR-23a-3p	-3.25416	0.01536	ATCACATTGCCAGGGATTTCC
hsa-miR-30a-3p	-4.72925	1.30E-07	CTTTCAGTCGGATGTTTGCAGC
hsa-miR-30a-5p	-7.69272	3.18E-07	TGTAAACATCCTCGACTGGAAG
hsa-miR-30c-2-3p	-5.80039	0.028767	CTGGGAGAAGGCTGTTTACTCT
hsa-miR-3158-3p	2.179298	0.003177	AAGGGCTTCCTCTCTGCAGGAC
hsa-miR-372-3p	7.10648	4.32E-06	AAAGTGCTGCGACATTTGAGCGT
hsa-miR-378a-3p	2.695553	0.000885	ACTGGACTTGGAGTCAGAAGGC
hsa-miR-483-3p	3.922906	0.031633	TCACTCCTCTCCTCCCGTCTT
hsa-miR-483-5p	4.78546	5.57E-05	AAGACGGGAGGAAAGAAGGGAG
hsa-miR-584-5p	-5.99766	0.03238	TTATGGTTTGCCTGGGACTGAG
hsa-miR-92b-3p	-4.26701	0.00015	TATTGCACTCGTCCCGGCCTCC

Table 2 Differential miRNA targets genes

miRNA_ID	Targets	n
hsa-miR-1269a	ADORA1, CPLX2, MECR, ADAR, EXTL2, NCKAP5L, ZXDC, HSF4, ENGASE, CEMP1, TMEM231, CREBBP, PLIN4, WIPF3, TRMT9B, FOXRED2, PTGER3, LTBP3, KCNK16, INAVA, RBM24, OTOA, CELF5, ATP8B3, IL6ST, CDIP1, TGIF2, POC1B-GALNT4, RIMS4, RHOBTB1, TRRAP, PASK, CDC20, STON2, TP53I11, CHRDL2, BTD, H6PD, ANLN, C12orf43, MYCN, BRD1, CRYGN KCNH3, ZNF618, NFAM1, MAST2, KY, DLAT, TPR, GALNT4, WASL, CDC25B, VPS9D1, ZFHX3, TMEM115, PATZ1, CCT8L2, PCLO, SOX8, ATP11A, CLUH, ELAC2, MRGBP, SMG9, ROBO3, VWA1, WDR82, ZNF696, C1QTNF6, ALPK2, GIPC3, SDK1, N4BP1, SLC39A3, KLF15, LYL1, LHX3, METTL25, LOC101927262, MLPH, DNAI1, GSTO2, ACSM1, GPBAR1, DGKD, LOC107986805, IQANK1, DAPK2, NLRC3, CHST8, EMID1, LOC112268219, ZNF331	93
hsa-miR-584-5p	DHDDS, IL4I1	2
hsa-miR-30c-2-3p	AK1, IGFBP3, PARP3, CT45A5, CTDSPL, FAM227A, CT45A1, CT45A3, CT45A6, GAB4, ELMSAN1, FAM160A2, ZNF30, EPHA10, ECE1, ZNF84, RAI14, CCDC166, CHAMP1, C6orf132, FREM3, THRA, TFR2, SYT7, CABLES1, NOL4L, GPR161, KIAA2012, ATG4D, KCNK9, SIPA1L1, ADAP1, CT45A8, CT45A9, CT45A7 , LOC79999, LOC388436, MAPK4, CCR1, PCDHB6, ARMC7, XXYLT1, SLC22A14, ZSCAN22, KCNS1, LOC643802, SETD1B, ELAVL3, CSPG4, CHST3, CRK, KCNA10, MPHOSPH10, SPEG, SF3B3, ATG4B, KCNV1, KLHL20, SUSD6, ZDHHC17, DNAH1, PCDHB5, YTHDF1, AJAP1, PCDHB10, PCDHB11, PCDHB12, PCDHB13, PCDHB14, PCDHB2, PCDHB3, PCDHB7, PCDHB9, PCDHB8, WRNIP1, PCDHB16, NSD1, NOL12, TREML2, LRRC3, HUWE1, PRPF38A, TP53RK, SAMD10, CT45A2, MT1E, MT1M, KCNK3, P4HA2, ZNF467, SLC28A3, LOC102724624, IL17REL, DOCK2, ZDHHC11, C8orf88, LOC107987099, SUOX	98
hsa-miR-193b-5p	OR8J3, SH3D19, RPS19, ABCF1, SLC35E2B, CITED2, DNAI2, SLC35E2A, KIF21B, ZNF541, CACHD1, BRD1, ZNF677, GPR83, DLC1, DLG2, GOT1, KCNQ4, CCPG1, PIK3R2, TIMM44, SEPHS2, NLGN4Y, TNXB, ARHGAP27, GALNT12, FHOD3, CIC, UBE2S, EAF1, RNASEH2B, RPH3AL, MIB2, COQ8A, CIB4, C11orf91, GAREM1	37
hsa-miR-146a-5p	EARS2, GPATCH11, GPAT3	3
hsa-miR-181a-2-3p	GCGR, EPN1, GTF2A1L, STON1-GTF2A1L, ARHGEF26, REXO4, TJP1, ATXN2, MIER2, PRSS8, COPB2, SF3A1, CLEC4E, KIF26A, INO80D, USP53, MIIP, WDR46	18
hsa-miR-483-5p	UBE3A, ZBTB16, DTX2, EPN1, MAMSTR, ESRRG, PABPC4, TTC39C, NFKBIL1, NONO, HOPX, ZNF701, DNAJC27, RBM14, MAP4K4, ARFGAP2, UBAP2, LOC403312, SHROOM2, PTK2, SEC16B, CRYZL2P-SEC16B, GCNT1, HDAC2, NOVA2, TIMP2, CNTNAP1, NEURL1, HOXA11, NHLH2, MUC3A, CLDN16, NR2E3, GPATCH1, TMEM33, CTC1, TIMM29, MFSD6L, PAN3, THNSL1, VPS9D1, NKAIN4, FAM172A, DTHD1, RNF112, HS3ST1, GRIN2D, GNG8, ADRM1, PRDM15, LOC285500, UNC5A, PRKAG3, ROBO1, RNF180, RNF39, C1QTNF4, TBX5, CD27, SRL, PRCD, LOC390937, ZNF273	62
hsa-let-7b-5p	HMGCR, TRPM3, FOXB2, WDR62, BUB1B-PAK6, CMKLR1, GPR85, DICER1, SPHK2, TRRAP, RAB18, SOX5, PFKFB1, PAK6, HDAC6, PDP2, DNASE1, TAF5, IFT172, ZNF589, SLC35B4, RIMKLA, OTOP1, KMT5C, ZNF546, LRTOMT	26
hsa-miR-92b-3p	RPL28, ST20, SOAT1, UBXN1, MFGE8, IREB2, AURKA, PIAS2, NTN4, HTD2, KDSR, TLK1, HINT3, DNHD1, ARID3A, MYO3A, IGDCC4, CEP170B, RGS6	19
hsa-miR-146b-5p	BBS12, MSH5, INTS4, LOC101929322	4