Preliminary exploration of animal models of congenital choledochal cysts

Table 1 Primers of interleukin-6, signal transducer and activator of transcription 3, and GAPDH

Gene	Gene ID	Forward primers	Reverse primers
IL-6	3569	GGTGGGTGTGTCCTCATTCC	GGCATTGCATCCCTGAGTTG
STAT3	6774	GTGGGAAGAATCACGCCTTC	AGATCCTGCACTCTCTTCCG
GAPDH	2597	GAACGGGAAGCTCACTGG	GCCTGCTTCACCACCTTCT

IL: Interleukin; STAT3: Signal transducer and activator of transcription 3.

Table 2 Numbers of various degrees of bile duct dilation in each group

Group	No dilation	Mild dilation	Moderate dilation	Severe dilation
	(φ < 1 mm)	(1 mm ≤ φ < 3 mm)	(3 mm ≤ φ < 10 mm)	(φ ≥ 10 mm)
A	0	10	2	0
B	0	5	7	0
C	0	2	8	2
D	11	1	0	0
E-G	36	0	0	0

φ: The maximum diameter of rat bile duct.

Table 3 Biochemical indices of rats in each group

Biochemical indices	Group A	Group B	Group C	Group D	Group E	Group F	Group G
ALT (U/L)	61.55	29.30	37.40	35.20	37.15	24.80	35.90
	(45.95-78.90)	(20.85-50.68)	(27.13-51.25)	(30.65-46.60)	(33.53-50.68)	(10.13-32.70)	(29.03-41.27)
AST (U/L)	131.80	80.60	52.30	46.60	75.20	46.75	50.20
	(85.50-208.15)	(41.23-146.28)	(35.05-68.48)	(40.50-54.75)	(66.93-119.18)	(18.90-59.75)	(38.95-87.25)
TBIL (μmol/L)	12.90	82.90	0.20	0.40	0.35	0.50	0.70
	(9.48-27.85)	(68.45-98.70)	(0.20-0.55)	(0.23-0.50)	(0.30-0.58)	(0.40-0.70)	(0.60-0.80)
DBIL (μmol/L)	11.25	75.20	0.10	0.20	0.20	0.20	0.40
	(7.68-23.35)	(64.95-89.65)	(0.10-0.28)	(0.13-0.30)	(0.10-0.28)	(0.20-0.45)	(0.33-0.50)
GGT (μmol/L)	6.45	9.65	7.75	8.15	8.35	7.65	7.50
	(3.15-13.78)	(8.08-21.33)	(7.38-7.90)	(7.83-8.45)	(8.05-8.90)	(7.18-8.48)	(7.00-8.08)
TBA (μmol/L)	72.05	31.25	25.55	27.45	16.45	17.05	18.10
	(25.60-211.53)	(17.33-105.60)	(21.93-34.93)	(16.40-40.65)	(13.03-25.70)	(11.45-22.28)	(14.90-22.30)

ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TBIL: Total bilirubin; DBIL: Direct bilirubin; GGT: Gamma-glutamyltransferase; TBA: Total bile acids.