Clinical significance of PCR in Helicobacter pylori DNA detection in human gastric disorders

Table 1 Comparison of urease test, enzyme-linked immunosorbent assay and polymerase chain reaction for detection of Helicobacter Pylori in gastric mucosa

Diagnosis	No. of patients	Rapid urease test	ELISA-Hp-IgG	PCR-Hp
		No. (%)	No. (%)	No. (%)
Chronic superficial gastritis	40	28 (70)	34 (85)	37 (93)
Chronic atrophic gastritis	12	7 (58)	8 (67)	10 (83)
GU	16	13 (81)	13 (81)	14 (88)
DU	44	37 (84)	37 (84)	42 (96)
GC	42	24 (68)	33 (78)	37 (88)
Total	154	114 (74)	125 (81)	140 (91)

ELISA-Hp-IgG: Enzyme-linked immunosorbent assay for anti Helicobacter pylori-IgG; PCR-Hp: Polymerase chain reaction to amplify the specific Helicobacter pylori urease gene fragments.

Table 2 The age distribution of gastric mucosal Helicobacter Pylori infection with polymerase chain reaction

Age	No. studied	Positive number	Positive rate (%)
10-	5	3	60
20-	12	9	75
30-	19	15	79
40-	50	48	96
50-	42	40	95
≥ 60	26	25	96
Total	154	140	91

Table 3 Stratified analysis of Helicobacter Pylori infection with respect to tumor location

Site of tumors	No. studied	PCR-Hp positive
		No.	(%)
Gastric antral	15	14	-93
Gastric corpus	17	16	-94
Gastric cardia	5	4	-80
Esopheageal	5	3	-60
Total	42	37	-88

PCR-Hp: Polymerase chain reaction to amplify the specific Helicobacter pylori urease gene fragments.

Table 4 Stratified analysis of Helicobacter Pylori infection with respect to tumor histological type

Tumors	No. studied	Helicobacter pylori positive
		No.	(%)
Adenocarcinoma	30	29	-97
Myxoepithelioma	2	1	-50
Undifferentiated cancer	3	2	-67
Myoangiosarcoma	2	1	-50
Benign tumors	5	4	-80
Total	42	37	-88