Preparation and application of monoclonal antibodies against hepatitis C virus nonstructural proteins

Table 1 Immunochemistry analysis of liver disease samples (n = 51)

Diagnosis	HCV-infected					Co-infected with HCV and HBV					HBV-infected
	No.	HBsAg	HBcAg	NS3Ag	NS5Ag	No.	HBsAg	HBcAg	NS3Ag	NS5Ag	No.	HBsAg	HBcAg	NS3Ag	NS5Ag
CAC	3	0	0	2	2	14	13	8	10	11	9	9	6	0	0
SAFH	1	0	0	0	0	1	0	0	0	0	2	1	1	0	0
CFH	2	0	0	1	1	5	4	3	2	2	5	4	2	0	0
HCC	0	0	0	0	0	5	3	1	1	1	4	3	1	0	0
Total	6	0	0	3	3	25	20	12	13	14	20	17	10	0	0

HCV: Hepatitis C virus; HBV: Hepatitis B virus; CAC: Chronic active cirrhosis; SAFH: Sub-acute fulminant hepatitis; CFH: Chronic fulminant hepatitis; HCC: Hepatocellular carcinoma.

Table 2 Anti-NS3 monoclonal antibody cross-reactivity with other antigens

Monoclonal Antibodie	CP9	CP10	C11	C7	HBsAg	NS3Ag
2B6	0.075	0.081	0.124	1.591	0.079	2.378
2F3	0.074	0.085	0.096	0.089	0.161	2.233
3D8	0.091	0.082	0.086	0.078	0.136	2.139
3D9	0.076	0.081	0.081	0.079	0.113	2.350

Table 3 Anti-NS3 epitope analysis

Monoclonal Antibodie	Inhibition rate (%)
	2B6	2F3	3D8	3D9
2B6	100.0	0	0	0
2F3	0	100.0	97.9	101.9
3D8	30.0	103.6	100.0	110.0
3D9	36.0	93.5	93.0	0

Table 4 Anti-NS5 monoclonal antibody cross-reactivity with other antigens

Monoclonal Antibodie	CP9	CP10	C11	C7	HBsAg	NS3Ag	NS5Ag
8B2	0.065	0.054	0.078	0.088	0.098	0.098	2.325
6F11	0.055	0.053	0.076	0.078	0.097	0.088	1.987
4C6	0.065	0.067	0.078	0.090	0.085	0.102	2.165
7D9	0.070	0.072	0.085	0.092	0.070	0.103	2.100