TATA-box-binding protein-associated factor 15 is a novel biomarker that promotes cell proliferation and migration in gastrointestinal stromal tumor

Table 1 Clinicopathologic features of the collected paired gastrointestinal stromal tumors samples

Case No.	Sex	Age in yr	Location	Tumor size length × width × height in cm	Mitosis count, per 50 HFPs	Risk classification based on NIH
1	Male	74	Stomach	2.8 × 2.8 × 2.0	> 5	High
2	Female	24	Stomach	6.0 × 4.0 × 5.0	> 5	High
3	Male	69	Stomach	7.5 × 5.5 × 4.0	> 10	High
4	Male	46	Small intestine	6.5 × 5.0 × 4.0	> 5	High
5	Female	65	Small intestine	6.5 × 5.5 × 5.0	> 5	High
6	Female	61	Stomach	5.5 × 3.5 × 4.0	> 5	High
7	Female	49	Stomach	8.5 × 8.0 × 7.0	< 5	Intermediate
8	Male	77	Stomach	6.0 × 6.0 × 5.0	< 5	Intermediate
9	Female	54	Stomach	6.0 × 4.5 × 3.5	< 5	Intermediate
10	Female	43	Stomach	5.5 × 4.5 × 3.0	< 5	Intermediate
11	Male	70	Stomach	7.0 × 7.0 × 5.5	< 5	Intermediate
12	Female	55	Stomach	8.0 × 8.0 × 7.0	< 5	Intermediate
13	Male	63	Small intestine	5.0 × 3.5 × 3.0	< 5	Low
14	Male	67	Stomach	2.3 × 1.5 × 1.5	< 5	Low
15	Male	44	Stomach	3.0 × 2.7 × 1.3	< 5	Low
16	Female	49	Stomach	1.0 × 0.8 × 0.8	< 5	Very low
17	Female	66	Stomach	2.0 × 1.8 × 1.0	< 5	Very low
18	Female	57	Stomach	2.0 × 1.8 × 1.5	< 5	Very low

HPF: High-powered field; NIH: National Institutes of Health.

Table 2 The top 10 upregulated and downregulated proteins

Category	Accession	Protein name	FC,T vs N	P value
Up	P29400	COL4A5	3.129881192	0.001086699
	O94772	LY6H	2.995133385	0.012292301
	P05204	HMGN2	2.92528705	2.10765E-07
	O00479	HMGN4	2.900071252	3.22924E-09
	P20930	FLG	2.87152332	0.002555182
	P52926	HMGA2	2.687785701	0.008942412
	Q6UX72	B3GNT9	2.576105848	4.91404E-09
	Q92804	TAF15	2.456058544	1.54458E-05
	O95081	AGFG2	2.441304592	2.93054E-06
	P09936	UCHL1	2.157884674	7.498E-07
Down	Q96BQ1	FAM3D	0.225135868	0.022611851
	Q5DID0	UMODL1	0.242287446	0.00020958
	P25815	S100P	0.267045455	3.77116E-06
	P13640	MT1G	0.274242	0.0028833
	P42330	AKR1C3	0.290893518	6.40059E-07
	Q9UBU3	GHRL	0.293081189	0.000379102
	Q9NS71	GKN1	0.311488849	6.5931E-06
	O95994	AGR2	0.330784665	9.59846E-06
	P19075	TSPAN8	0.350779233	7.40703E-09
	P55087	AQP4	0.352590936	0.035544439

T: Tumor tissues; N: Matched tumor normal tissues; FC: Fold change.

Table 3 Correlation between the expression of TATA-box-binding protein-associated factor 15 and clinicopathological characteristics in gastrointestinal stromal tumors

Characteristics	TAF15 expression		P value
	Negative	Positive
Total number	31	130
Sex			0.2730a
Male	16 (9.9)	53 (32.9)
Female	15 (9.3)	77 (58.7)
Age in yr			0.8228a
≤ 60	15 (9.3)	60 (37.2)
> 60	16 (9.9)	70 (43.5)
Tumor size
Maximum diameter in cm
≤ 5	29 (18.0)	74 (46.0)	0.0001a
> 5	2 (1.2)	56 (34.8)
GIST risk stage			0.0021b
Very low risk	15 (9.3)	30 (18.6)
Low risk	11 (6.8)	31 (19.3)
Intermediate risk	3 (1.9)	29 (18.0)
High risk	2 (1.2)	40 (24.8)
Metastasis			0.1316a
-	31 (19.3)	121 (75.2)
+	0 (0.0)	9 (5.6)

^aP values were calculated by χ² test.

^bP values were calculated by Mann-Whitney U test.

Total patients of 161. Data are presented as n(%). TAF15: TATA-box-binding protein-associated factor 15; GIST: Gastrointestinal stromal tumor.