Basic Study
Copyright ©The Author(s) 2022.
World J Gastroenterol. Sep 7, 2022; 28(33): 4787-4811
Published online Sep 7, 2022. doi: 10.3748/wjg.v28.i33.4787
Table 1 List of primary and secondary antibodies used in immunofluorescent staining
Antibody name
Species
Dilution
Catalog number
Company
Primary antibodies
CK8Mouse 1:200904801Biolegend
CK19Rabbit1:200ab15463Abcam
CD44Mouse 1:100sc-7297Santa Cruz Biotechnology
Ki67Mouse 1:50sc-23900Santa Cruz Biotechnology
p- HistoneH2AX Rabbit1:200#9718SCell Signaling
Secondary antibodies
Alexa fluoro 488 Goat anti-mouse1:200A-28175Invitrogen
Alexa fluoro 568Goat anti-rabbit1:200A-11011Invitrogen
Phalloidin1:200R415Invitrogen
Table 2 List of primary and secondary antibodies used in western blot experiments
Antibody name
Species
Dilution
Catalog number
Company
Primary antibodies
p53Rabbit 1:50sc-6243Santa Cruz Biotechnology
p21Rabbit 1:1000sc-2947Cell Signaling
CD133Rabbit1:500#64326SCell Signaling
β-cateninMouse1:200sc-7963Santa Cruz Biotechnology
PCNAMouse1:50sc-25280Santa Cruz Biotechnology
p-ERKMouse1:300sc-7383Santa Cruz Biotechnology
ERKRabbit1:300sc-93Santa Cruz Biotechnology
p-AKTRabbit1:1000#4060Cell Signaling
AKTRabbit1:1000#4691Cell Signaling
GAPDH-HRP (6C5)Mouse1:20,000#MAB5476Abnova
Secondary antibodies
Goat anti-mouse Goat1:1000sc-516102Santa Cruz Biotechnology
Mouse anti-rabbitMouse1:1000sc-2357Santa Cruz Biotechnology