Duodenal-jejunal bypass increases intraduodenal bile acids and upregulates duodenal SIRT1 expression in high-fat diet and streptozotocin-induced diabetic rats

doi:10.3748/wjg.v28.i31.4338

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Aug 21, 2022; 28(31): 4338-4350
Published online Aug 21, 2022. doi: 10.3748/wjg.v28.i31.4338

Table 1 Primer sequence

Gene	Forward (5’-3’)	Reverse (5’-3’)
SIRT1	GGGAACCTCTGCCTCATCTACAT	CGCCACCTAACCTATGACACAA
AMPK	GAAGATCGGACACTACGTGCT	ACTGCCACTTTATGGCCTGTC
FXR	CATTACAACGCGCTCACCTG	CCCATCTCTCTGCACTTCCT
TGR5	TACTCACAGGGTTGGCACTG	CAAAAGTTGGGGGCCAAGTG
SHP	TCCGGTCCCCAGCATACTTA	GAAAGACTCCAGGCAGTGCT
GAPDH	GATTTGGCCGTATCGGAC	GAAGACGCCAGTAGACTC

FXR: Farnesoid X receptor; SHP: Small heterodimer partner; TGR5: Takeda G-protein-coupled receptor 5.

Citation: Han HF, Liu SZ, Zhang X, Wei M, Huang X, Yu WB. Duodenal-jejunal bypass increases intraduodenal bile acids and upregulates duodenal SIRT1 expression in high-fat diet and streptozotocin-induced diabetic rats. World J Gastroenterol 2022; 28(31): 4338-4350
URL: https://www.wjgnet.com/1007-9327/full/v28/i31/4338.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i31.4338