Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics

Table 1 List of differential protein numbers

Compare group	Up-regulated	Down-regulated	All-regulated
M/N	111	91	202
HM/M	41	76	117
EA/M	59	86	145

N: Normal group; M: Dextran sulfate sodium-induced ulcerative colitis model group; HM: Herb-partitioned moxibustion group; EA: Electroacupuncture group.

Table 2 Differentially expressed proteins in dextran sulfate sodium-induced ulcerative colitis model group rats that regulated by herb-partitioned moxibustion

Protein ID	Description	Symbol	M/N		HM/M
			Mean ratio	Q value	Mean ratio	Q value
Q02563	Synaptic vesicle glycoprotein 2A	Sv2a	4.42	0.024	0.25	0.021
A0A0G2JZN1	Immunoglobulin kappa chain variable 13-85	Igkv13-85	4.07	0.020	0.37	0.028
Q56A27	Nuclear cap-binding protein subunit 1	Ncbp1	2.47	0.018	0.56	0.004
Q91W30	Aldose reductase-like protein	Akr1b8	1.85	0.006	0.63	0.017
Q63223	Rat hemoglobin beta-chain (Fragment)	Rat hemoglobin beta-chain (Fragment)	1.57	0.005	0.46	0.004
P62243	40S ribosomal protein S8	Rps8	1.56	0.006	0.83	0.023
P06399	Fibrinogen alpha chain	Fga	1.51	0.003	0.75	0.004
O55215	Ribosomal protein S2	Rps2-ps6	1.45	0.003	0.80	0.004
P07756	Carbamoyl-phosphate synthase (ammonia), mitochondrial	Cps1	1.33	0.009	0.70	0.004
P11232	Thioredoxin	Txn	1.28	0.002	0.74	0.004
Q6PDU7	ATP synthase subunit g, mitochondrial	ATP5L	1.27	0.028	0.76	0.004
P10888	Cytochrome c oxidase subunit 4 isoform 1, mitochondrial	Cox4i1	1.26	0.002	0.80	0.004
P31399	ATP synthase subunit d, mitochondrial	Atp5h	1.34	0.003	0.77	0.004
Q6P756	Adaptin ear-binding coat-associated protein 2	Necap2	1.48	0.011	0.83	0.023
P19511	ATP synthase F (0) complex subunit B1, mitochondrial	Atp5f1	1.24	0.003	0.83	0.004
A0A0G2K950	3’-phosphoadenosine 5’-phosphosulfate synthase 2	Papss2	1.27	0.002	0.74	0.004
A0A0G2K5E7	ATP-citrate synthase	Acly	1.35	0.017	0.83	0.030
A0A0G2QC59	RCG45649, isoform CRA_a	Spout1	0.54	0.013	2.13	0.011
A0A0G2JWX9	Chloride channel accessory 1	Clca1	0.50	0.002	1.25	0.004
Q99N82	Colon SVA-like protein	Sval1	0.73	0.028	6.82	0.004
B5DFG4	Heat shock 27kD protein family, member 7 (cardiovascular)	Hspb7	0.76	0.016	1.24	0.050
D3ZSF3	Peptidyl-prolyl cis-trans isomerase	-	0.75	0.003	1.40	0.013
P51647	Retinal dehydrogenase 1	Aldh1a1	0.81	0.002	1.22	0.004
Q05820	Putative lysozyme C-2	Lyz2	0.67	0.013	1.48	0.050
F1LWF2	Doublecortin-like kinase 3	Dclk3	0.24	0.005	4.67	0.011

N: Normal group; M: Dextran sulfate sodium-induced ulcerative colitis model group; HM: Herb-partitioned moxibustion group; ATP5L: ATP synthase subunit g; Atp5f1: ATP synthase beta subunit precursor; Sv2a: Synaptic vesicle glycoprotein 2A; Ncbp1: Nuclear cap binding protein subunit 1; Cps1: Carbamoyl phosphate synthetase 1; Cox4i1: Cytochrome c oxidase subunit 4 isoform 1; Dclk3: Doublecortin like kinase 3; Akr1b8: Aldo-keto reductase family 1 member B8; RpS8: Ribosomal protein S8.

Table 3 Differentially expressed proteins in dextran sulfate sodium-induced ulcerative colitis model group rats that regulated by electroacupuncture

Protein ID	Description	Symbol	M/N		EA/M
			Mean ratio	Q value	Mean ratio	Q value
Q02563	Synaptic vesicle glycoprotein 2A	Sv2a	4.42	0.024	0.15	0.013
Q56A27	Nuclear cap-binding protein subunit 1	Ncbp1	2.47	0.018	0.55	0.003
Q63223	Rat hemoglobin beta-chain (Fragment)	-	1.57	0.005	0.38	0.002
P06399	Fibrinogen alpha chain	Fga	1.51	0.003	0.76	0.002
P07756	Carbamoyl-phosphate synthase (ammonia), mitochondrial	Cps1	1.33	0.009	0.69	0.004
Q6PDU7	ATP synthase subunit g, mitochondrial	ATP5L	1.27	0.028	0.82	0.002
P10888	Cytochrome c oxidase subunit 4 isoform 1, mitochondrial	Cox4i1	1.26	0.002	0.74	0.002
P19511	ATP synthase F (0) complex subunit B1, mitochondrial	Atp5f1	1.51	0.003	0.81	0.002
A0A0G2K0D3	Leiomodin-1	Lmod1	1.13	0.003	0.77	0.002
A0A0G2QC59	RCG45649, isoform CRA_a	Spout1	0.54	0.013	1.88	0.002
B2RZ27	SH3 domain binding glutamic acid-rich protein-like 3	Sh3bgrl3	0.74	0.027	1.31	0.027
D3ZSF3	Peptidyl-prolyl cis-trans isomerase	-	0.75	0.003	1.47	0.003
Q31266	MHC class I RT1.Aw3 protein	-	0.81	0.029	1.44	0.005
G3V826	Transketolase OS = rattus norvegicus	Tkt	0.71	0.003	1.23	0.002
F1LWF2	Doublecortin-like kinase 3	Dclk3	0.24	0.005	4.09	0.002

N: Normal group; M: Dextran sulfate sodium-induced ulcerative colitis model group; EA: Electroacupuncture group; ATP5L: ATP synthase subunit g; Atp5f1: ATP synthase beta subunit precursor; Sv2a: Synaptic vesicle glycoprotein 2A; Ncbp1: Nuclear cap binding protein subunit 1; Cps1: Carbamoyl phosphate synthetase 1; Cox4i1: Cytochrome c oxidase subunit 4 isoform 1; Dclk3: Doublecortin like kinase 3.

Table 4 The Kyoto Encyclopedia of Genes and Genomes pathway related to immunity and inflammation with differentially expressed proteins between normal group and dextran sulfate sodium-induced ulcerative colitis model group

Pathway name	Protein ID	Number	P value
Primary immunodeficiency	M0RDF2, IGG2B, M0RA79, Q4QQW0, F1LXY6, Q569B3	6	0.003
Nuclear factor-kappa B signalling pathway	M0RDF2, IGG2B, M0RA79, Q4QQW0, F1LXY6, Q569B3, B2RZB2	7	0.021
Natural killer cell mediated cytotoxicity	GRZ2, M0RDF2, IGG2B, M0RA79, Q4QQW0, F1LXY6, Q569B3	7	0.025
Intestinal immune network for IgA production	M0RDF2, IGG2B, HB2B, M0RA79, Q8VI32, Q4QQW0, F1LXY6, Q569B3, B2RZB2	9	4.25E-05
Fc gamma R-mediated phagocytosis	M0RDF2, Q6AYB2, IGG2B, E9PU64, M0RA79, Q4QQW0, F1LXY6, B2GV73, Q569B3	9	0.007
Complement and coagulation cascades	CFAI, FIBB, M0RBJ7, PLMN, A1L114, CO9, A1M, FIBG, FIBA, Q99N82, A0A0G2JY31	11	8.29E-06

N: Normal group; M: Dextran sulfate sodium-induced ulcerative colitis model group; IgA: Immunoglobulin A.

Table 5 The Kyoto Encyclopedia of Genes and Genomes pathway related to immunity, inflammation and oxidative phosphorylation with differentially expressed proteins between herb-partitioned moxibustion group and dextran sulfate sodium-induced ulcerative colitis model group

Pathway name	Protein ID	Number	P value
Primary immunodeficiency	IGG2C, Q5BJZ2, F1LXY6, Q569B3	4	0.009
Oxidative phosphorylation	ATP5H, ATPO, COX41, AT5F1, Q5UAJ5, NMES1, ATP5L	7	0.002
Nitrogen metabolism	CAH1, CAH3, CPSM	3	0.001
Natural killer cell mediated cytotoxicity	GRZ2, IGG2C, Q5U1Y2, Q5BJZ2, F1LXY6, Q569B3	6	0.005
Intestinal immune network for IgA production	IGG2C, Q5BJZ2, Q6MG98, F1LXY6, Q569B3	5	0.002
Fc gamma R-mediated phagocytosis	IGG2C, Q5U1Y2, Q5BJZ2, F1LXY6, Q569B3	5	0.040
Complement and coagulation cascades	Q5M7T5, Q99N82, Q7TQ70, FIBA	4	0.031
B cell receptor signalling pathway	IGG2C, Q5U1Y2, Q5BJZ2, F1LXY6, Q569B3	5	0.020

M: Dextran sulfate sodium-induced ulcerative colitis model group; HM: Herb-partitioned moxibustion group; IgA: Immunoglobulin A; ATP5L: ATP synthase subunit g.

Table 6 The Kyoto Encyclopedia of Genes and Genomes pathway related to immunity, inflammation and oxidative phosphorylation with differentially expressed proteins between electroacupuncture group and dextran sulfate sodium-induced ulcerative colitis model group

Pathway name	Protein ID	Number	P value
PI3K-Akt signalling pathway	F1M6Q3, D4A3K7, P70570, Q5M7V3, A0A096P6L8, Q5BJZ2, F1LPR6, GBB4, PCKGC	9	0.042
Oxidative phosphorylation	B2RYT5, CX7A2, D4A565, CX6C2, COX41, M0RA24, AT5F1, B2RYS8, G3V8S4, D3ZFQ8, NMES1, ATP5L, ATPK	13	3.42E-07
Intestinal immune network for IgA production	Q5M7V3, Q5BJZ2, F1LPR6, Q6MG98	4	0.032
Calcium signalling pathway	GNAS2, Q5M7V3, A0A0G2K059, Q5BJZ2, F1LPR6, A0A0G2JSR0, ADT2	7	0.027

M: Dextran sulfate sodium-induced ulcerative colitis model group; EA: Electroacupuncture group; IgA: Immunoglobulin A; ATP5L: ATP synthase subunit g; PI3K-Akt: Phosphatidylinositol 3-kinase protein kinase B; COX41: Cytochrome c oxidase subunit IV isoform 1; PCKGC: Phosphoenolpyruvate carboxykinase 1; ATPK: ATP synthase membrane subunit F; ADT2: Arogenate dehydratase 2.