Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress

Table 1 The demographic and clinical data of subjects

Group	CHB	Control	HCC
n	34	8	8
Age (years; 25%, 75%)	41.00 (35.00, 48.75)	32.50 (28.25, 41.85)	47.00 (40.25, 53.42)
ALT (U/L; 25%, 75%)	137.50 (59.00, 221.50)	31.5000 (14.5000, 63.1725)	48.50 (39.00, 65.25)
TBil (μmol/L; 25%, 75%)	16.300 (11.500, 24.225)	10.80 (9.42, 20.58)	20.6 (14.5, 32.5)
AFP (ng/mL; 25%, 75%)	4.5400 (2.9850, 13.1725)	2.02 (1.05, 4.75)	179.80 (14.65, 207.53)

CHB: Chronic hepatitis B; HCC: Hepatocellular carcinoma; AFP: Alpha-fetoprotein; ALT: Serum alanine aminotransferase; TBil: Total bilirubin.

Table 2 The sequences of short hairpin RNAs

Insert content			5'-3'
Mouse	Afp shRNA	Target sequence	GCATCCATTGCAAAGGAATTA
		shRNA sequence	GCATCCATTGCAAAGGAATTACGAATAATTCCTTTGCAATGGATGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAACGAATTCTCCGAACGTGTCACGTTT
Human	PERK shRNA	Target sequence	GCACTTTAGATGGGAGAATTG
		shRNA sequence	GCACTTTAGATGGGAGAATTGCGAACAATTCTCCCATCTAAAGTGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAACGAATTCTCCGAACGTGTCACGTTT
	ATF6 shRNA	Target sequence	GCAGGTCCTCCTGTTATTAGA
		shRNA sequence	GCAGGTCCTCCTGTTATTAGACGAATCTAATAACAGGAGGACCTGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAA CGAATTCTCCGAACGTGTCACGTTT
	AFP shRNA	Target sequence	GCTTCCATATTGGATTCTTAC
		shRNA sequence	GCTTCCATATTGGATTCTTAC CGAAGTAAGAATCCAATATGGAAGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAA CGAATTCTCCGAACGTGTCACGTTT

AFP: Alpha-fetoprotein; ATF6: Activating transcription factor-6; PERK: Protein kinase R-like ER kinase; shRNA: Short hairpin RNA.