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©The Author(s) 2021.
World J Gastroenterol. Dec 14, 2021; 27(46): 7982-7994
Published online Dec 14, 2021. doi: 10.3748/wjg.v27.i46.7982
Published online Dec 14, 2021. doi: 10.3748/wjg.v27.i46.7982
Table 1 Quantitative real time PCR primer sequences
| Gene | Forward (5’-3’) | Reverse (5’-3’) |
| hnf1b | CCCATCCTCAAAGAGCTCCA | AGAGGTGGGATTGGTTCAGG |
| GAPDH(Mouse) | ACTCCACTCACGGCAAATTC | TCTCCATGGTGGTGAAGACA |
| miR-217-5p | UACUGCAUCAGGAACUGACUGGA | mRQ3' Primer (Takara, Kyoto, Japan) |
| U6 | Takara, Kyoto, Japan | Takara, Kyoto, Japan |
Table 2 Values of the evaluation indexes
| Body weight loss on day 7 (%) (mean ± SD) | DAI on day 7 (mean ± SD) | Colon length (mean ± SD) | Macroscopic scores (mean ± SD) | Histopathological scores (mean ± SD) | n | |
| Water + PBS | 125.30 ± 6.30 | 0.00 ± 0.00 | 9.70 ± 0.56 | 0.00 ± 0.00 | 0.00 ± 0.00 | 5 |
| DSS + PBS | 89.11 ± 8.02 | 5.20 ± 0.45 | 6.58 ± 0.48 | 7.8 ± 1.30 | 13.00 ± 2.55 | 5 |
| DSS + rSj16 | 106.00 ± 5.97 | 1.40 ± 0.89 | 8.12 ± 0.35 | 3.20 ± 0.84 | 4.4 ± 1.14 | 5 |
- Citation: Zhang LC, Wu XY, Yang RB, Chen F, Liu JH, Hu YY, Wu ZD, Wang LF, Sun X. Recombinant protein Schistosoma japonicum-derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis. World J Gastroenterol 2021; 27(46): 7982-7994
- URL: https://www.wjgnet.com/1007-9327/full/v27/i46/7982.htm
- DOI: https://dx.doi.org/10.3748/wjg.v27.i46.7982