Association of gene and protein expression and genetic polymorphism of CC chemokine ligand 4 in colorectal cancer

Table 1 Genotypic and allelic distributions of the CC chemokine ligand 4 gene polymorphism (rs10491121) in n (%), among 610 patients with colorectal cancer and 409 healthy controls

Genotype/allele	Patients	Controls
G/G	(250 (41.0)	176 (43.0)
G/A	281 (46.1)	181 (44.3)
A/A	79 (12.9)	52 (12.7)
G	781 (64.0)	533 (65.2)
A	439 (36.0)	285 (34.8)

CCL4: CC chemokine ligand 4.

Table 2 Genotypic and allelic distributions of the CC chemokine ligand 4 gene polymorphism (rs10491121) in n (%), regarding tumour location and disease stage among patients with colon cancer

Variable	Cases	Genotype			Allele
Right colon		G/G	G/A	A/A	G	A
Stages I + II	116	38 (32.8)	62 (53.4)	16 (13.8)	138 (59.5)	94 (40.5)
Stages III + IV	78	44 (56.4)b	24 (30.8)	10 (12.8)	112 (71.8)a	44 (28.2)
Left colon
Stages I + II	67	23 (34.3)	34 (50.8)	10 (14.9)	80 (59.7)	54 (40.3)
Stages III + IV	75	27 (36.0)	37 (49.3)	11 (14.7)	91 (60.7)	59 (39.3)

^aP < 0.05.

^bP < 0.01. CCL4: CC chemokine ligand 4.

Table 3 Genotypic and allelic distributions of the CCL4 gene polymorphism (rs10491121) in n (%), regarding non-mucinous and mucinous cancer among patients (n = 610) with colorectal cancer

Genotype/allele	Non-mucinous	Mucinous
G/G	222 (42.0)	28 (34.1)
G/A	247 (46.8 )	34 (41.5)
A/A	59 (11.2 )	20 (24.4)b
G	691 (65.4)	90 (54.9)
A	365 (34.6)	74 (45.1)b

^bP < 0.01.

CCL4: CC chemokine ligand 4.

Table 4 Tissue levels and plasma levels of CC chemokine ligand 4 in colorectal cancer patients

Variable	Cases, n	CCL4 protein
Tissue
Colorectal cancer tissue	98	137 [5–3413] pg/mgb
Paired normal tissue	98	72 [5–2205] pg/mg
Plasma
Patient	103	63 [8–3428] pg/mLa
Control	97	57 [2–178] pg/mL

Data are shown as median [range].

^aP < 0.05.

^bP < 0.001. CCL4: CC chemokine ligand 4.

Table 5 Disease stage in relation to cancer tissue levels of CC chemokine ligand 4 in colorectal cancer patients

Variable	Cases, n	CCL4 protein in pg/mg
Stage
I	18	262 [12–3326]
II	33	188 [5–2797]
III	22	125 [24–3413]
IV	25	71 [10–1188]b
I + II	51	235 [5–3326]
III + IV	47	84 [10–3413]b

Data are shown as median [range].

^bP < 0.001. CCL4: CC chemokine ligand 4.