Human immune repertoire in hepatitis B virus infection

Table 1 Immune repertoire investigation of hepatitis B virus infection

Number	Ref.	Patients	Amounts of samples	Repertoire	Sequencing reads	Tools
1	Deulofeut et al[59], 1997	2 volunteers	2	TCR repertoire	-	PRISM
2	Höhn et al[60], 2002	Healthy individuals immunized with recombinant hepatitis B surface antigen	7	TCR-VA and -VB repertoire in CD8+ T cells	-	FITC/PE
3	Soroosh et al[69], 2003	Nine healthy adult responders and six nonresponders	30	TCR beta chain variable gene		FRAGMENT MANAGER’ software program
4	Yang et al[41], 2011	Forty-two patients with CSHB	42	TCR CDR3	-	SPSS 16.0 software
5	Xiong et al[44], 2014	18 patients with CSHB and 8 controls	26	TCR Vβ repertoire	-	DNAMAN software version 1.0
6	Han et al[52], 2015	Liver cancers and healthy adults	160	TCR CDR3	495708702	BLAST
7	Galson et al[62], 2015	Naïve group: n = 9; vaccine group: n = 9	108	B-cell repertoire	365863	R
8	Qu et al[37], 2016	4 CHB patients before and after HBeAg seroconversion	8	TCR Vβ repertoire	370210 to 685596	Homemade Perl script
9	Yang et al[55], 2016	12 HBeAg SC patients and 20 no HBeAg SC patients	96	TCRBV families	-	SPSS software version 19.0
10	Galson et al[63], 2016	9 healthy subjects	63	B-cell repertoire		R
11	Chang et al[70], 2016	4 pairs of HBeAg positive carrier and HBsAg negative non-carrier siblings	16	IgG immune repertoire	2.2 million	PEAR, Python, Numpy and SciPy, Ipython
12	Ma et al[64], 2017	3 healthy volunteers	6	IgM and IgG H chain CDR3 repertoires		IMGT/High V-Quest
13	Jiang et al[36], 2018	3 pairs of healthy identical twins and 7 pairs of chronic hepatitis B patients	20	CD8+ T-cell receptor beta (TCRβ) chains	50 million	MIXCR
14	Yan et al[49], 2019	6 patients with HBV-related ACLF and 6 controls	12	BCR CDR3 region	An average number of 12243860.30 in the control group and an average number of 1229965.30 in the ACLF group	GraphPad Prism software
15	Miyasaka et al[61], 2019	5 volunteers	30	T-cell and B-cell receptor repertoire	TCR β chain repertoire was 153151 before HB vaccination, 180093 after the second HB vaccination, and 129044 the third HB vaccination; BCR IgG heavy (H) chain repertoires were 106664 before HB vaccination, 126237 after the second HB vaccination, and 135663 the third HB vaccination	SPSS software package, Easy R (EZR) version 1.37, GraphPad software package
16	Shen et al[48], 2020	5 HBV-ACLF	20	TCR repertoire	163259321	MIXCR
17	Lian et al[57], 2020	20 CHB patients undergoing 1-yr ETV treatment (10 HBeAg SC patients and 10 no HBeAg SC patients)	60	T-cell repertoire	-	MIXCR

ACLF: Acute-on-chronic liver failure; BCR: B-cell receptor; CDR3: Complementary determining region 3; CSHB: Chronic severe hepatitis B; ETV: Entecavir; HBV: Hepatitis B virus; Ig: Immunoglobulin. SC: Seroconversion; TCR: T-cell receptor.

Table 2 Specific TRB family and clinical significance

TRB family	Clinical significance
TRBVβ2	Development and treatment of CHB
TRBVβ3	HBeAg seroconversion
TRBVβ5	Severity of CHB
TRBVβ7	Development and treatment of CHB, severity of CHB
TRBVβ9	Severity of CHB
TRBVβ11	HBeAg seroconversion, severity of CHB
TRBVβ12	Development and treatment of CHB, HBeAg seroconversion, severity of CHB
TRBVβ14	HBeAg seroconversion
TRBVβ18	Severity of CHB
TRBVβ20	HBeAg seroconversion
TRBVβ24	HBeAg seroconversion
TRBVβ28	Development and treatment of CHB

CHB: Chronic hepatitis B; HBeAg: Hepatitis B e antigen.