Resveratrol alleviates intestinal mucosal barrier dysfunction in dextran sulfate sodium-induced colitis mice by enhancing autophagy

Table 1 Assessment of the disease activity index

Body mass loss (%)	Stool consistency	Bleeding	Score
< 2%	Normal	No bleeding	0
≥ 2 - < 5%	-	-	1
≥ 5 - < 10%	Loose stools	Slight bleeding	2
≥ 10 - < 15%	-	-	3
≥ 15	Watery diarrhea	Gross bleeding	4

Table 2 Histological scoring of colitis

Features	Grade	Description
Inflammation	0	None
	1	Slight
	2	Moderate
	3	Severe
Extent	0	None
	1	Mucosa
	2	Mucosa and submucosa
	3	Transmural
Regeneration	4	No tissue repair
	3	Surface epithelium not intact
	2	Regeneration with crypt depletion
	1	Almost complete regeneration
	0	Complete regeneration or normal tissue
Crypt damage	0	None
	1	Basal 1/3 damaged
	2	Basal 2/3 damaged
	3	Only surface epithelium intact
	4	Entire crypt and epithelium lost
Percent involvement	1	1%-25%
	2	26%-50%
	3	51%-75%
	4	76%-100%

Table 3 ZO-1 and occludin expressions in colitis

Group	ZO-1 expression		χ2/P value	Occludin expression		χ2/P value
	Low (%)	High (%)		Low (%)	High (%)
Control	6 (50.0%)	6 (50.0%)	8.812/0.029	7 (58.3%)	5 (41.7%)	10.987/0.010
DSS	7 (77.8%)	2 (22.2%)		8 (88.9%)	1 (11.1%)
DSS + 5-ASA	7 (63.6%)	4 (36.4%)		9 (81.8%)	2 (18.2%)
DSS + RES	2 (16.7%)	10 (83.3%)		3 (25.0%)	9 (75.0%)

DSS: Dextran sulfate sodium; 5-ASA: 5-aminosalicylic acid; RES: Resveratrol.