Copyright
©The Author(s) 2020.
World J Gastroenterol. Apr 28, 2020; 26(16): 1912-1925
Published online Apr 28, 2020. doi: 10.3748/wjg.v26.i16.1912
Published online Apr 28, 2020. doi: 10.3748/wjg.v26.i16.1912
Table 1 Local gene expression of pro and anti-inflammatory cytokines in paired inflamed vs non-inflamed tissues of inflammatory bowel disease patients and paired tumoral vs adjacent non-tumoral mucosa of colorectal cancer patients
Genes | IBD | CRC | P value IBD vs CRC | ||||
dCt ± SD | RQ (min-max) | dCt ± SD | RQ (min-max) | ||||
Inflamed tissue | Non-inflamed tissue | Tumoral tissue | Non-tumoral tissue | ||||
Foxp3 | 14.94 ± 2.65 | 15.81 ± 2.59 | 1.82 (0.11-74.85) | 18.62 ± 5.5 | 21.30 ± 4.3 | 6.42 (0.47-97.2) | 0.025 |
TGFB1 | 9.52 ± 4.13 | 8.97 ± 4.4 | 0.68 (0.03-2.27) | 17.27 ± 2.9 | 18.63 ± 1.9 | 2.37 (0.211-93.7) | 0.007 |
IL-10 | 16.87 ± 1.76 | 17.7 ± 1.5 | 1.78 (0.22-28.5) | 16.43 ± 5.5 | 19.17 ± 4.4 | 6.66 (0.43-202.3) | 0.023 |
IL-6 | 17.13 ± 3.05 | 19.53 ± 2.7 | 5.28 (0.27-61.6) | 19.11 ± 3.8 | 21.07 ± 2.5 | 3.88 (0.03-512.4) | 0.784 |
IL-23A | 18.0 ± 2.09 | 18.1 ± 2.8 | 1.07 (0.07-6.61) | 12.56 ± 2.1 | 17.55 ± 2.5 | 28.5 (2.04-219.5) | 0.0003 |
IL-17A | 18.9 ± 2.65 | 20.3 ± 2.9 | 2.70 (0.07-20.5) | 24.7 ± 4.3 | 26.9 ± 3.0 | 4.69 (0.034-494.9) | 0.574 |
Table 2 The local gene expression in inflammatory bowel disease patients compared to that in early colorectal cancer cases
Genes | IBD | Early stages CRC (1 + 2) | P value IBD vs Early CRC | ||||
dCt ± SD | RQ (min-max) | dCt ± SD | RQ (min-max) | ||||
Inflamed tissue | Non-inflamed tissue | Tumoral tissue | Non-tumoral tissue | ||||
Foxp3 | 14.94 ± 2.65 | 15.81 ± 2.59 | 1.82 (0.11-74.85) | 16.98 ± 5.8 | 20.41 ± 4.5 | 10.74 (0.615-97.2) | 0.023 |
TGFB1 | 9.52 ± 4.13 | 8.97 ± 4.4 | 0.68 (0.03-2.27) | 16.99 ± 2.4 | 18.83 ± 2.3 | 3.59 (0.222-93.7) | 0.057 |
IL-10 | 16.87 ± 1.76 | 17.7 ± 1.5 | 1.78 (0.22-28.5) | 16.17 ± 5 | 19.25 ± 4.1 | 8.44 (1.253-202.3) | 0.019 |
IL-6 | 17.13 ± 3.05 | 19.53 ± 2.7 | 5.28 (0.27-61.6) | 18.12 ± 4.7 | 20.59 ± 1.9 | 5.55 (0.03-512.4) | 0.97 |
IL-23A | 18.0 ± 2.09 | 18.1 ± 2.8 | 1.07 (0.07-6.61) | 11.69 ± 1.9 | 17.65 ± 2.9 | 61.93 (2.04-219.5) | 0.001 |
IL-17A | 18.9 ± 2.65 | 20.3 ± 2.9 | 2.70 (0.07-20.5) | 22.42 ± 3.6 | 27.17 ± 2.9 | 26.83 (2.73-494.9) | 0.029 |
Table 3 The local gene expression in inflammatory bowel disease patients compared to that in advanced colorectal cancer cases
Genes | IBD | Advanced stages CRC (3 + 4) | P value IBD vs Advanced CRC | ||||
dCt ± SD | RQ (min-max) | dCt ± SD | RQ (min-max) | ||||
Inflamed tissue | Non-inflamed tissue | Tumoral tissue | Non-tumoral tissue | ||||
Foxp3 | 14.94 ± 2.65 | 15.81 ± 2.59 | 1.82 (0.11-74.85) | 19.71 ± 5.2 | 21.89 ± 4.2 | 4.55 (0.45-45.1) | 0.101 |
TGFB1 | 9.52 ± 4.13 | 8.97 ± 4.4 | 0.68 (0.03-2.27) | 17.46 ± 3.3 | 18.5 ± 1.6 | 2.05 (0.211-14.56) | 0.176 |
IL-10 | 16.87 ± 1.76 | 17.7 ± 1.5 | 1.78 (0.22-28.5) | 16.69 ± 6.2 | 19.09 ± 5 | 5.26 (0.43-158.8) | 0.113 |
IL-6 | 17.13 ± 3.05 | 19.53 ± 2.7 | 5.28 (0.27-61.6) | 19.78 ± 3.1 | 21.39 ± 2.8 | 3.05 (0.025-434.7) | 0.608 |
IL-23A | 18.0 ± 2.09 | 18.1 ± 2.8 | 1.07 (0.07-6.61) | 13.64 ± 2.1 | 17.44 ± 2.4 | 13.96 (3.93-36.28) | 0.012 |
IL-17A | 18.9 ± 2.65 | 20.3 ± 2.9 | 2.70 (0.07-20.5) | 26.62 ± 3.9 | 26.73 ± 3.2 | 1.07 (0.034-89.26) | 0.350 |
Table 4 Serum levels of pro and anti-inflammatory cytokines in inflammatory bowel disease and colorectal cancer patients
Cytokines | IBD patients | CRC patients | P value (U-test) |
TGFB1 (ng/mL) | 11.19 (9.36-20.99) | 22.57 (18.08-29.65) | 0.0008 |
IL-10 (pg/mL) | 0.4 (0.0-2.8) | 8.29 (6.75-15.12) | 0.000001 |
IL-6 (pg/mL) | 0.0 (0.0-2.7) | 3.21 (2.03-4.19) | 0.012 |
IL-23A (pg/mL) | 0.7 (0.0-3.7) | 27.55 (25.35-33.0) | 0.000001 |
IL-17A (pg/mL) | 0.0 (0.0-0.26) | 2.85 (0.0-9.0) | 0.034 |
Table 5 Serum levels of anti and pro-inflammatory cytokines in inflammatory bowel disease patients compared to early and advanced colorectal cancer cases
Cytokines | IBD | Early CRC | P value (U-test), IBD vs early CRC | Advanced CRC | P value (U-test), IBD vs advanced CRC |
TGFB1 (ng/mL) | 11.19 (9.36-20.99) | 22.74 (18.17-27.98) | 0.001344 | 21.51 (15.5-31.05) | 0.0031 |
IL-10 (pg/mL) | 0.4 (0.0-2.8) | 7.73 (6.0-12.71) | 0.000006 | 8.84 (7.2-19.81) | 0.000002 |
IL-6 (pg/mL) | 0.0 (0.0-2.7) | 3.06 (2.03-4.19) | 0.0153 | 4.19 (2.15-8.4) | 0.044 |
IL-23A (pg/mL) | 0.7 (0.0-3.7) | 25.7 (24.6-32.5) | 0.000024 | 30.5 (26.3-33.5) | 0.000009 |
IL-17A (pg/mL) | 0.0 (0.0-0.26) | 3.62 (0.0-8.23) | 0.0086 | 1.2 (0.0-12.08) | 0.178 |
- Citation: Velikova TV, Miteva L, Stanilov N, Spassova Z, Stanilova SA. Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer. World J Gastroenterol 2020; 26(16): 1912-1925
- URL: https://www.wjgnet.com/1007-9327/full/v26/i16/1912.htm
- DOI: https://dx.doi.org/10.3748/wjg.v26.i16.1912