MiR-96-5p inhibition induces cell apoptosis in gastric adenocarcinoma

Table 1 Distribution of characteristics in gastric adenocarcinoma patients and healthy subjects

Variables	Patients, n = 20		Controls, n = 20		P value1
	n	%	n	%
Age in yr, mean ± SD	62.1 ± 5.2		60.1 ± 10.2		0.73
Weight in kg, mean ± SD	64.0 ± 7.1		69.1 ± 6.6		0.88
Gender
Male	13	65.0	1	55.0	0.64
Female	7	35.0	9	45.0
Depth of invasion
T1/T2	7	35.0
T3/T4	13	65.0
Lymph node metastasis
N0	6	30.0
N1/N2/N3	14	70.0
Distant metastasis
M0	8	40.0
M1	12	60.0
TNM stage
I/II	7	35.0
III/IV	13	65.0

Data are presented as n (%) unless otherwise indicated.

¹Independent-samples t-test and two-sided χ² test for selected variables distributions between cases and controls. SD: Standard deviation.

Table 2 Primers used for the quantitative real-time polymerase chain reaction

Gene	Primer sequence
miR-96-5p	F:5’-TCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAGCAAAAA-3’
	R: 5’- ACACTCCAGCTGGGTTTGGCACTAGCACATT-3’
miR-125a-5p	F: 5’- CCCTGAGACCCTTTAACCT-3’
	R: 5’- GTCCAGTTTTTTTTTTTTTTTCACAG-3’
miR-145-3p	F: 5’- GGTCCAGTTTTCCCAGGA-3’
	R: 5’- CCAGTTTTTTTTTTTTTTTAGGGATTC-3’
miR-222-5p	F: 5’- GCTCAGTAGCCAGTGTAGA-3’
	R: 5’- GTCCAGTTTTTTTTTTTTTTTAGGATCT-3’
miR-379-3p	F: 5’- GCAGTGGTAGACTATGGAAC-3’
	R: 5’- GGTCCAGTTTTTTTTTTTTTTTCCT-3’
miR-652-5p	F: 5’- CCTAGGAGAGGGTGCCA-3’
	R: 5’- GTCCAGTTTTTTTTTTTTTTTGAATGG-3’
miR-708-3p	F: 5’- GCAACTAGACTGTGAGCTTC-3’
	R: 5’- GGTCCAGTTTTTTTTTTTTTTTCTAGA-3’
GAPDH	F: 5’-AGAAGGCTGGGGCTCATTTG-3’
	R: 5’-AGGGGCCATCCACAGTCTTC-3’
U6	F: 5’-AGGGGCCATCCACAGTCTTC-3’
	R: 5’-AACGCTTCACGAATTTGCGT-3’

GAPDH: Glyceraldehyde-phosphate dehydrogenase.