Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Aug 28, 2019; 25(32): 4696-4714
Published online Aug 28, 2019. doi: 10.3748/wjg.v25.i32.4696
Table 1 Primers used for amplification of selective intestinal microbiota
MicroorganismBase sequence
LactobacillusPrimer F5'-ACGGGAGGCAGCAGTAGGGA-3'
Primer R5'-AGCCGTGACTTTCTGGTTGATT-3'
BifidobacteriumPrimer F5'-GATTCTGGCTCAGGATGAACGC-3'
Primer R5'-CTGATAGGACGCGACCCCAT-3'
Faecalibacterium_prausnitziiPrimer F5'-AGATGGCCTCGCGTCCGA-3'
Primer R5'-CCGAAGAACCTTCTTCCTCC-3'
Escherichia_coliPrimer F5'-CATGCCGCGTGTATGAAGAA-3'
Primer R5'-CGGGTAACGTCAATGAGCAAA-3'
Table 2 Primers used for amplification of NLRP6, ASC, Caspase-1, and GAPH mRNA
mRNABase sequence
NLRP6Primer A5'-AAGGTGAAGGAGAGGAATG-3'
Primer B5'-GAAGAGCCGATTGAAAGTG-3'
ASCPrimer A5'-TGCTGGATGCTCTGTATGG-3'
Primer B5'-CAAGTAGGGCTGTGTTTGC-3'
Caspase-1Primer A5'-GACAAGCCCAAGGTTATC-3'
Primer B5'-GGCCTTCTTAATGCCATC-3'
IL-1βPrimer A5'-AAGGTGAAGGAGAGGAAT-3'
Primer B5'-GAAGAGCCGATTGAAAGTG-3'
IL-18Primer A5'-TGCTGGATGCTCTGTATGG-3'
Primer B5'-CAAGTAGGGCTGTGTTTGC-3'
GAPDHPrimer A5'-GTCGGTGTGAACGGATTTG-3'
Primer B5'-TCCCATTCTCAGCCTTGAC-3'
Table 3 Correlations between selective intestinal microbes and mRNA and protein expression of NLRP6, ASC, and Caspase-1
Lactobacillus
Bifidobacterium
Faecalibacterium_prau-snitzii
Escherichia_coli
rPrPrPrP
NLRP6 mRNA0.5500.0000.5420.0000.6240.000-0.4950.000
ASC mRNA-0.6410.000-0.6330.000-0.7470.0000.4320.005
Caspase-1 mRNA-0.6560.000-0.6880.000-0.7370.0000.7160.000
NLRP6 protein0.6390.0000.7500.0000.9200.000-0.6260.000
ASC protein-0.6070.000-0.7090.000-0.7970.0000.6060.000
Caspase-1 protein-0.6540.000-0.6690.000-0.7490.0000.5010.001