Copyright
©The Author(s) 2018.
World J Gastroenterol. Feb 28, 2018; 24(8): 949-956
Published online Feb 28, 2018. doi: 10.3748/wjg.v24.i8.949
Published online Feb 28, 2018. doi: 10.3748/wjg.v24.i8.949
Table 1 Overview of primers and probe sequences for all PCR methods
Primers (5’-3’) | Sequence | Product size (bp) |
Reverse transcription PCR | ||
SYT-sense | CCAGCAGAGGCCTTATGGA | 118 |
SSX2-antisense | GCACAGCTCTTTCCCATCA | |
Genomic PCR | ||
SYT1-sense | GTAGTTTGACCGGCTGCAGAA | |
SYT2-sense | GGTGGTCTGGTTTGTTCACC | |
SYT3-sense | GCAAATGTTTATTGAGCAACCA | |
SYT4-sense | GGGAGAAATTAAAAGGGTGGA | |
SYT5-sense | CACCTGTGAAACCATCAGCA | |
SYT6-sense | TTTTCTTTATGGATTATGCTTTTGG | |
SYT7-sense | GCTTACTAGGAGTTTCATTGTAATTG | |
SYT8-sense | CAGCCTGATAAACTGTATACC | |
SYT9-sense | GATTTGAATGCGTGATCACAAG | |
SYT10-sense | GATTGGATTCCAGACATTGTG | |
SYT11-sense | GCCACCTTGGAATTTGTTAATG | |
SYT12-sense | CTGATGATTGAAGAAACCGAG | |
SSX2-1-antisense | ACGGAGAATCAGGGTTCTTTGG | |
SSX2-2-antisense | TCAGTCTCCACACTGGCAAC | |
SSX2-3-antisense | TCAAGGCAACATCCGACTCC | |
SSX2-4-antisense | TGGTTTCCAGGGATAGAATGCT | |
Real-time PCR | ||
SYT-specific-sense | CCAGCAACAGTAGTTTACTTTCTATC | 106 |
SSX2-specific-antisense | AAACATAGGGAGGCGACAAA | |
SYT-SSX2-FAM probe-sense | ATA+CAA+T+C+CA+G+CAG1 |
Table 2 Results of rt-PCR analysis by the specific probe and primer sets
Types of DNA | Sample | Ct value 1 | Ct value 2 | Mean |
Genomic | Normal tissue | Undetermined | Undetermined | Undetermined |
Tumor tissue | 31.94651 | 31.30283 | 31.62467 | |
β-actin | Tumor tissue | 28.97511 | 30.95062 | 29.96287 |
- Citation: Ogino S, Konishi H, Ichikawa D, Hamada J, Shoda K, Arita T, Komatsu S, Shiozaki A, Okamoto K, Yamazaki S, Yasukawa S, Konishi E, Otsuji E. Detection of fusion gene in cell-free DNA of a gastric synovial sarcoma. World J Gastroenterol 2018; 24(8): 949-956
- URL: https://www.wjgnet.com/1007-9327/full/v24/i8/949.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i8.949