Case Report
Copyright ©The Author(s) 2018.
World J Gastroenterol. Feb 28, 2018; 24(8): 949-956
Published online Feb 28, 2018. doi: 10.3748/wjg.v24.i8.949
Table 1 Overview of primers and probe sequences for all PCR methods
Primers (5’-3’)SequenceProduct size (bp)
Reverse transcription PCR
SYT-senseCCAGCAGAGGCCTTATGGA118
SSX2-antisenseGCACAGCTCTTTCCCATCA
Genomic PCR
SYT1-senseGTAGTTTGACCGGCTGCAGAA
SYT2-senseGGTGGTCTGGTTTGTTCACC
SYT3-senseGCAAATGTTTATTGAGCAACCA
SYT4-senseGGGAGAAATTAAAAGGGTGGA
SYT5-senseCACCTGTGAAACCATCAGCA
SYT6-senseTTTTCTTTATGGATTATGCTTTTGG
SYT7-senseGCTTACTAGGAGTTTCATTGTAATTG
SYT8-senseCAGCCTGATAAACTGTATACC
SYT9-senseGATTTGAATGCGTGATCACAAG
SYT10-senseGATTGGATTCCAGACATTGTG
SYT11-senseGCCACCTTGGAATTTGTTAATG
SYT12-senseCTGATGATTGAAGAAACCGAG
SSX2-1-antisenseACGGAGAATCAGGGTTCTTTGG
SSX2-2-antisenseTCAGTCTCCACACTGGCAAC
SSX2-3-antisenseTCAAGGCAACATCCGACTCC
SSX2-4-antisenseTGGTTTCCAGGGATAGAATGCT
Real-time PCR
SYT-specific-senseCCAGCAACAGTAGTTTACTTTCTATC106
SSX2-specific-antisenseAAACATAGGGAGGCGACAAA
SYT-SSX2-FAM probe-senseATA+CAA+T+C+CA+G+CAG1
Table 2 Results of rt-PCR analysis by the specific probe and primer sets
Types of DNASampleCt value 1Ct value 2Mean
GenomicNormal tissueUndeterminedUndeterminedUndetermined
Tumor tissue31.9465131.3028331.62467
β-actinTumor tissue28.9751130.9506229.96287