Copyright
©The Author(s) 2018.
World J Gastroenterol. Feb 21, 2018; 24(7): 775-793
Published online Feb 21, 2018. doi: 10.3748/wjg.v24.i7.775
Published online Feb 21, 2018. doi: 10.3748/wjg.v24.i7.775
Table 1 Criteria of inclusion for patients in each group
Patient group | Periodic Follow-up (n) | New stadiation of IBD (n) | Abdominal pain (n) | Chronic diarrhea (n) | Colorectal cancer screening (n) | Stipsis (n) | Hemorrhoids (n) |
UC | 16 | 2 | - | - | - | - | - |
CD | 2 | 8 | - | - | - | - | - |
CTRL | 31 | - | 5 | 4 | 2 | 1 | 1 |
Table 2 Number of biopsies analyzed by immunohistochemistry in each tract of large intestine
Patient group | Biopsy status | Large | Intestine | ||||
Proximal | Tract | Distal | Tract | ||||
Cecum colon (n) | Ascending colon (n) | Transverse colon (n) | Descending colon (n) | Sigmoid colon (n) | Rectum (n) | ||
UC | Inflamed | 2 | 6 | 4 | 6 | 7 | 7 |
Non-Inflamed | 7 | 9 | 5 | 6 | 3 | 3 | |
CD | Inflamed | 0 | 4 | 3 | 4 | 4 | 4 |
Non-Inflamed | 1 | 1 | 0 | 1 | 2 | 1 | |
CTRL | Inflamed | 2 | 4 | 3 | 2 | 4 | 4 |
Non-Inflamed | 7 | 5 | 6 | 6 | 7 | 7 |
Table 3 Primary and secondary antibodies for immunohistochemical analysis
Antibody | Host | Dilution | Source |
Anti-GLUT2 | Goat | 1:200 | cat#ab111117, abcam, Cambridge, United Kingdom |
Anti-GLUT5 | Rabbit | 1:400 | cat#ab36057, abcam |
Anti-SGLT1 | Rabbit | 1:200 | cat#ab14686, abcam |
Anti-LYVE-1 | Rabbit | 1:500 | cat#Bs-1311R, Bioss,Woburn, MA, United States |
Anti-VEGF | Rabbit | 1:200 | cat#RB-222, Thermo Scientific, Fremont, CA, United States |
Polyclonal anti-rabbit IgG/biotinylated | Swine | 1:400 | cat#E0353, Dako, Milan, Italy |
Polyclonal anti-goat IgG/biotinylated | Rabbit | 1:400 | cat#E0466, Dako |
CyTM3-Fab fragment anti-rabbit IgG | Goat | 1:100 | cat#111-167, Jackson Lab., Baltimore, MD, United States |
FITC-fab fragment anti-rabbit IgG | Goat | 1:100 | cat#111-097, Jackson Lab. |
Unconjugated fab fragment anti-rabbit IgG | Goat | 1:30 | cat#111-007, Jackson Lab. |
Table 4 Percentage of samples with GLUT2, SGLT1 and GLUT5 expression in epithelial cells of large intestine biopsies
Patient group | Large | Intestine | ||||||
Biopsy status | Proximal | Tract | Biopsy status | Distal | Tract | |||
GLUT2, % | SGLT1, % | GLUT5, % | GLUT2, % | SGLT1, % | GLUT5, % | |||
UC | Inflamed (n = 12) | 8 | 9 | 17 | Inflamed (n = 20) | 0 | 15 | 15 |
Non-inflamed (n = 21) | 5 | 5 | 33 | Non-inflamed (n = 12) | 8 | 8 | 17 | |
CD | Inflamed (n = 7) | 0 | 0 | 0 | Inflamed (n = 12) | 8 | 8 | 0 |
Non-inflamed (n = 2) | 0 | 0 | 0 | Non-inflamed (n = 4) | 0 | 0 | 0 | |
CTRL | Inflamed (n = 9) | 11 | 0 | 33 | Inflamed (n = 10) | 30 | 10 | 0 |
Non-inflamed (n = 18) | 17 | 0 | 17 | Non-inflamed (n = 20) | 20 | 1 | 15 |
Table 5 Percentage of samples with GLUT5 expression in clusters of lymphatic vessels in large intestine biopsies
Patient group | Large | Intestine | ||
Proximal | Tract | Distal | Tract | |
Biopsy status | GLUT5, % | Biopsy status | GLUT5, % | |
UC | Inflamed (n = 12) | 50.0 | Inflamed (n = 20) | 55.0 |
Non-Inflamed (n = 21) | 47.6 | Non-Inflamed (n = 12) | 83.3 | |
CD | Inflamed (n = 7) | 71.0 | Inflamed (n = 12) | 83.0 |
Non-Inflamed (n = 2) | 50.0 | Non-Inflamed (n = 4) | 25.0 | |
CTRL | Inflamed (n = 9) | 55.5 | Inflamed (n = 10) | 60.0 |
Non-Inflamed (n = 18) | 44.4 | Non-Inflamed (n = 20) | 85.0 |
Table 6 Percentage of samples with GLUT5-immunoreactive clusters of vessels in biopsy groups subdivided by patient body mass index
Patient group | Biopsy status | Large | Intestine | ||||
Proximal | Tract | Distal | Tract | ||||
Patient | BMI | Patient | BMI | ||||
Normal weight, % | Over-weight, % | Obese, % | Normal weight, % | Over-weight, % | Obese, % | ||
UC | Inflamed | 71 | 20 | 0 | 70 | 43 | 33 |
Non-inflamed | 57 | 25 | 50 | 87 | 100 | 0 | |
CD | Inflamed | 80 | 50 | 0 | 87 | 75 | 0 |
Non-inflamed | 100 | 0 | 0 | 50 | 0 | 0 | |
CTRL | Inflamed | 80 | 25 | 0 | 67 | 50 | 0 |
Non-inflamed | 22 | 50 | 100 | 82 | 80 | 100 |
- Citation: Merigo F, Brandolese A, Facchin S, Missaggia S, Bernardi P, Boschi F, D’Incà R, Savarino EV, Sbarbati A, Sturniolo GC. Glucose transporter expression in the human colon. World J Gastroenterol 2018; 24(7): 775-793
- URL: https://www.wjgnet.com/1007-9327/full/v24/i7/775.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i7.775