Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats

Table 1 Organ weights following the 14-d treatment of male rats with acetic acid-induced gastric ulcers with lansoprazole and hydroalcoholic extract from the leaves of Eugenia punicifolia

	Treatment
	Vehicle	Lansoprazole	HEEP
Organ weights, g
Heart	3.15 ± 0.03	3.13 ± 0.03	3.14 ± 0.06
Kidneys	4.81 ± 0.05	4.79 ± 0.04	4.84 ± 0.05
Lung	3.62 ± 0.08	3.73 ± 0.10	3.59 ± 0.06
Liver	11.56 ± 0.18	11.55 ± 0.10	11.26 ± 0.13
Spleen	2.94 ± 0.02	2.98 ± 0.05	2.99 ± 0.04
Biochemical parameters
Glucose, mg/dL	150.10 ± 6.04	170.90 ± 4.73a	154.70 ± 3.80
Creatinine, mg/dL	0.34 ± 0.02	0.34 ± 0.02	0.33 ± 0.01
Urea, mg/dL	54.75 ± 3.15	52.13 ± 1.71	54.13 ± 1.94
γ-GT, U/L	< 1.0	< 1.0	< 1.0
AST, U/L	137.80 ± 18.43	163.60 ± 22.33	144.50 ± 10.76
ALT, U/L	42.88 ± 3.55	48.88 ± 3.67	46.25 ± 3.09

Vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Data were collected at the end of treatment. Results are expressed as the means ± standard error of the mean for each group (n = 7-8). Statistical significance was determined using one-way ANOVA followed by Dunnett's test.

^aP < 0.05 vs vehicle; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; γ-GT: Gamma-glutamyl transpeptidase. HEEP: Hydroalcoholic extract from the leaves of Eugenia punicifolia.

Table 2 Organ weights following the 14-d treatment of intact female rats with acetic acid-induced gastric ulcers with lansoprazole and hydroalcoholic extract from the leaves of Eugenia punicifolia

	Treatment
	Vehicle	Lansoprazole	HEEP
Organ weights, g
Heart	3.43 ± 0.05	3.50 ± 0.03	3.44 ± 0.06
Kidneys	4.74 ± 0.03	4.81 ± 0.04	4.69 ± 0.06
Lung	4.23 ± 0.07	4.53 ± 0.06	4.01 ± 0.30
Liver	10.67 ± 0.14	10.90 ± 0.10	10.61 ± 0.09
Spleen	2.56 ± 0.06	2.56 ± 0.07	2.48 ± 0.06
Biochemical parameters
Glucose, mg/dL	119.10 ± 3.27	116.90 ± 3.21	122.30 ± 3.34
Creatinine, mg/dL	0.45 ± 0.02	0.41 ± 0.02	0.45 ± 0.01
Urea, mg/dL	49.50 ± 3.36	53.29 ± 1.11	53.70 ± 3.20
γ-GT, U/L	< 1.0	< 1.0	< 1.0
AST, U/L	202.70 ± 12.73	198.40 ± 18.84	162.10 ± 7.24
ALT, U/L	57.00 ± 3.40	60.57 ± 3.59	65.86 ± 2.79

Vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Data were collected at the end of treatment. Results are expressed as the means ± standard error of the mean for each group (n = 7-8). Statistical significance was determined using one-way ANOVA followed by Dunnett's test. No significant differences vs vehicle were noted; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; γ-GT: Gamma-glutamyl transpeptidase. HEEP: Hydroalcoholic extract from the leaves of Eugenia punicifolia.

Table 3 Organ weights following the 14-d treatment of ovariectomized rats with acetic acid-induced gastric ulcers with lansoprazole and hydroalcoholic extract from the leaves of Eugenia punicifolia

	Treatment
	Vehicle	Lansoprazole	HEEP
Organ weights, g
Heart	3.35 ± 0.04	3.39 ± 0.05	3.49 ± 0.09
Kidneys	4.52 ± 0.07	4.56 ± 0.07	4.61 ± 0.05
Lung	4.28 ± 0.11	4.30 ± 0.10	4.47 ± 0.17
Liver	10.56 ± 0.16	10.61 ± 0.10	10.60 ± 0.11
Spleen	2.59 ± 0.06	2.68 ± 0.08	2.65 ± 0.05
Biochemical parameters
Glucose, mg/dL	117.30 ± 4.36	126.70 ± 4.48	119.00 ± 5.46
Creatinine, mg/dL	0.42 ± 0.00	0.43 ± 0.01	0.43 ± 0.01
Urea, mg/dL	55.08 ± 2.24	48.97 ± 1.67	55.37 ± 2.92
γ-GT, U/L	< 1.0	< 1.0	< 1.0
AST, U/L	176.80 ± 15.37	192.70 ± 14.77	191.00 ± 21.04
ALT, U/L	62.17 ± 3.68	57.57 ± 2.36	56.43 ± 1.92

Vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Data were collected at the end of treatment. Results are expressed as the means ± standard error of the mean for each group (n = 7-8). Statistical significance was determined using one-way ANOVA followed by Dunnett's test. No significant differences vs vehicle were noted; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; γ-GT: Gamma-glutamyl transpeptidase. HEEP: Hydroalcoholic extract from the leaves of Eugenia punicifolia.