Copyright
©The Author(s) 2018.
World J Gastroenterol. Sep 28, 2018; 24(36): 4178-4185
Published online Sep 28, 2018. doi: 10.3748/wjg.v24.i36.4178
Published online Sep 28, 2018. doi: 10.3748/wjg.v24.i36.4178
Table 1 Designations and sequences of primers
Designation (mice) | Sequence (5’-3’) |
GAPDH | F: GGCATCCTGACCCTCAAGTA |
R: GGGGTGTGAACCTCTCAAA | |
Collagen I | F: GGACACTACTGGATCGACCTAAC |
R: CTCACCTGTCTCCATGTTGCA | |
Collagen III | F: CTACCTTGCTCAGTCCTATGAGTCTAGA |
R: TCCCGAGTCGCAGACACATAT | |
Wnt1 | F: GAAACCGCCGCTGGAACT |
R: CCCTGCCTCGTTATTGTGAAG | |
β-catenin | F: ACC TCC CAAGTC CTG TAT |
R: CCT GGT CCT CGT CAT TTA |
Table 2 Biochemical indices of blood and liver
Groups | HA (μg/L) | Hyp (μg/g) |
C | 61 ± 20.6bcde | 195.6 ± 10.5bcde |
CCl4 | 157.3 ± 44.3acde | 503 ± 31.7acde |
V | 70.2 ± 12.9b | 375.2 ± 26.9ab |
P | 79.5 ± 11.6b | 361.3 ± 24.1ab |
V + P | 65 ± 12.1b | 353.7 ± 21.6ab |
Table 3 The mRNA levels of collagen I, collagen III, Wnt1, and β-catenin of liver
Groups | Collagen I | Collagen III | Wnt1 | β-catenin |
C | 1.17 ± 0.16bcde | 0.62 ± 0.15bcde | 0.56 ± 0.13bcde | 0.48 ± 0.21bcde |
CCl4 | 4.73 ± 0.76acde | 2.47 ± 1.12acde | 1.77 ± 0.32acde | 2.16 ± 0.42acde |
V | 1.97 ± 0.31b | 1.29 ± 0.19ab | 1.06 ± 0.35ab | 1.25 ± 0.33ab |
P | 1.75 ± 0.46b | 1.35 ± 0.17ab | 0.83 ± 0.19ab | 0.71 ± 0.46ab |
V + P | 1.53 ± 0.12abcd | 1.03 ± 0.08abcd | 0.61 ± 0.24abcd | 0.59 ± 0.14abcd |
- Citation: Huang GR, Wei SJ, Huang YQ, Xing W, Wang LY, Liang LL. Mechanism of combined use of vitamin D and puerarin in anti-hepatic fibrosis by regulating the Wnt/β-catenin signalling pathway. World J Gastroenterol 2018; 24(36): 4178-4185
- URL: https://www.wjgnet.com/1007-9327/full/v24/i36/4178.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i36.4178