Copyright
©The Author(s) 2018.
World J Gastroenterol. Jan 14, 2018; 24(2): 226-236
Published online Jan 14, 2018. doi: 10.3748/wjg.v24.i2.226
Published online Jan 14, 2018. doi: 10.3748/wjg.v24.i2.226
Table 1 Characteristics of patients with hepatitis C virus-1b infection
Variable | High expression of miR-93-5p | Low expression of miR-93-5p | P value |
Gender | 0.382 | ||
Male | 25 | 20 | |
Female | 17 | 22 | |
Age | 0.189 | ||
Median | |||
>44 | 19 | 26 | |
≤44 | 23 | 16 | |
HCV-1b treatment | 0.001 | ||
Resistance | 25 | 9 | |
Sensitivity | 17 | 33 |
Table 2 Primers sequences
mRNA | Sequence |
β-actin forward | 5’-TGTCCACCTTCCAGCAGATGT-3’ |
β-actin reverse | 5’-TGTCACCTTCACCGTTCCAGTT-3’ |
IFNAR1 forward | 5’-TGGTGACAGCGTGAGACTCTT-3’ |
IFNAR1 reverse | 5’-GCAGTAGCCAGCAGCATCAG-3’ |
MiR-93-5p forward | 5′- GCCGCCAAAGTGCTGTTC-3′ |
MiR-93-5p reverse | 5′-CAGAGCAGGGTCCGAGGTA-3′ |
U6 forward | 5′-CAGCACATATACTAAAATTGGAACG-3′ |
U6 reverse | 5′-ACGAATTTGCGTGTCATCC-3′ |
Table 3 Receiver operating characteristics curve analysis of serum miR-93-5p concentration
AUC | Sensitivity | Specificity | Cut-off value (amol/μL) |
0.8846 (Infection vs Healthy) | 0.7619 | 1 | 0.009774 |
0.8562 (Sensitivity vs Healthy) | 0.7000 | 1 | 0.009774 |
0.9265 (Infection vs Resistance) | 0.8529 | 1 | 0.010870 |
0.8359 (Resistance vs Sensitivity) | 0.7647 | 1 | 0.030300 |
- Citation: He CL, Liu M, Tan ZX, Hu YJ, Zhang QY, Kuang XM, Kong WL, Mao Q. Hepatitis C virus core protein-induced miR-93-5p up-regulation inhibits interferon signaling pathway by targeting IFNAR1. World J Gastroenterol 2018; 24(2): 226-236
- URL: https://www.wjgnet.com/1007-9327/full/v24/i2/226.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i2.226