Copyright
©The Author(s) 2018.
World J Gastroenterol. Apr 28, 2018; 24(16): 1766-1778
Published online Apr 28, 2018. doi: 10.3748/wjg.v24.i16.1766
Published online Apr 28, 2018. doi: 10.3748/wjg.v24.i16.1766
Score | Weight loss, % | Stool consistency | Bloodstain or gross bleeding |
0 | None | Normal | Negative |
1 | 1-5 | - | - |
2 | 5-10 | Loose stool | Positive |
3 | 10-15 | - | - |
4 | > 15 | Diarrhea | Gross bleeding |
Score | Inflammation | Depth of lesions | Destruction of crypt | Width of lesions, % |
0 | None | None | None | |
1 | Slight | Mucosa | Basal 1/3 damaged | 1-25 |
2 | Moderate | Mucosa and submucosa | Basal 2/3 damaged | 26-50 |
3 | Severe | Transmural | Intact epithelium only | 51-75 |
4 | - | - | Total crypt and epithelium | 76-100 |
Gene | Primer sequences | Amplification length |
TNF-α | Forward: 5’-GCCTATGTCTCAGCCTCTTCTC-3’ | 22 |
Reverse: 5’-TGGTGGTTTGCTACGACGTG-3’ | 20 | |
CXCL-1 | Forward: 5’-TCACCTCAAGAACATCCAGAGC-3’ | 22 |
Reverse: 5’-ACTTGGGGACACCTTTTAGCAT-3’ | 22 | |
IL-6 | Forward: 5’-TCTCTGCAAGAGACTTCCATCC-3’ | 22 |
Reverse: 5’-TTCCACGATTTCCCAGAGAACA-3’ | 22 | |
β-actin | Forward: 5’-AGATCAAGATCATTGCTCCTCC-3’ | 22 |
Reverse: 5’-GGTGTAAAACGCAGCTCAGTAA-3’ | 22 |
- Citation: Gong SS, Fan YH, Wang SY, Han QQ, Lv B, Xu Y, Chen X, He YE. Mucosa repair mechanisms of Tong-Xie-Yao-Fang mediated by CRH-R2 in murine, dextran sulfate sodium-induced colitis. World J Gastroenterol 2018; 24(16): 1766-1778
- URL: https://www.wjgnet.com/1007-9327/full/v24/i16/1766.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i16.1766