Basic Study
Copyright ©The Author(s) 2018.
World J Gastroenterol. Apr 28, 2018; 24(16): 1766-1778
Published online Apr 28, 2018. doi: 10.3748/wjg.v24.i16.1766
Table 1 Criteria for disease activity index
ScoreWeight loss, %Stool consistencyBloodstain or gross bleeding
0NoneNormalNegative
11-5--
25-10Loose stoolPositive
310-15--
4> 15DiarrheaGross bleeding
Table 2 Histological score to quantify the degree of colitis
ScoreInflammationDepth of lesionsDestruction of cryptWidth of lesions, %
0NoneNoneNone
1SlightMucosaBasal 1/3 damaged1-25
2ModerateMucosa and submucosaBasal 2/3 damaged26-50
3SevereTransmuralIntact epithelium only51-75
4--Total crypt and epithelium76-100
Table 3 Primer sequences and amplification length
GenePrimer sequencesAmplification length
TNF-αForward: 5’-GCCTATGTCTCAGCCTCTTCTC-3’22
Reverse: 5’-TGGTGGTTTGCTACGACGTG-3’20
CXCL-1Forward: 5’-TCACCTCAAGAACATCCAGAGC-3’22
Reverse: 5’-ACTTGGGGACACCTTTTAGCAT-3’22
IL-6Forward: 5’-TCTCTGCAAGAGACTTCCATCC-3’22
Reverse: 5’-TTCCACGATTTCCCAGAGAACA-3’22
β-actinForward: 5’-AGATCAAGATCATTGCTCCTCC-3’22
Reverse: 5’-GGTGTAAAACGCAGCTCAGTAA-3’22