Evaluation of safety for hepatectomy in a novel mouse model with nonalcoholic-steatohepatitis

Table 1 Serum parameters of normal liver and nonalcoholic steatohepatitis groups after partial hepatectomy

	6 h after PH				12 h after PH
	AST	ALT	T-Bil	IL-6	AST	ALT	T-Bil	IL-6
Normal 70%PH	2343.3 ± 6160.4	1828.3 ± 990.4	1.43 ± 0.5	2036.0 ± 1470.9	2976.7 ± 1395.7	2053.3 ± 886.2	2.0 ± 0.9	731.5 ± 483.7
NASH 30%PH	1610.0 ± 3700.6	1507.1 ± 563.5	0.76 ± 0.2	1511.5 ± 284.8	1841.3 ± 619.1	1522.9 ± 537.9	0.9 ± 0.7	692.8 ± 211.1
NASH 70%PH	3064.3 ± 1289.8a	2422.9 ± 1194.8	2.57 ± 1.36b	3026.2 ± 2127.5	4067.5 ± 2059.2a	2403.8 ± 1111.8	3.85 ± 0.96b	987.9 ± 550.7

Data are presented as the mean ± SD (n = 5-7).

^aP < 0.05,

^bP < 0.01 vs other groups. NASH: Nonalcoholic steatohepatitis; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; PH: Partial hepatectomy; T-Bil: Total bilirubin; IL-6: Interleukin-6.

Table 2 Gene expression microarray

Function	Gene name	Gene abbreviation	Fold-change (> 1.4)	P value (< 0.05)
Regulate the cellular concentrations of cyclic nucleotides	Phosphodiesterase 4B, cAMP-specific	PDE4B	2.102691211	0.0063
Growth arrest	Growth arrest and DNA-damage-inducible, alpha	GADD45A	1.672778704	0.013
Signal transduction	Coronin 1C	CORO1C	1.489851976	0.031
Stimulates expression of cytokines, including IL6, MIF and VEGFA	Hypoxia inducible lipid droplet associated	HILPDA	1.481465541	0.011
EGF-like growth factor	Heparin binding EGF-like growth factor	HBEGF	1.432049736	0.043
Cell-cell junctions	Membrane associated guanylate kinase, WW And PDZ domain containing 1	MAGI1	1.430522907	0.0222
Innate immune system	Ankyrin repeat and SOCS box containing 13	ASB13	0.515175325	0.0042
Apoptosis and autophagy	TIA1 cytotoxic granule-associated RNA binding protein-like 1	TIAL1	0.609948905	0.027
Gene expression	Nucleic acid binding protein 1	NABP1	0.6558797325	0.042
Cell cycle	S-phase kinase-associated protein 2, E3 ubiquitin protein ligase	SKP2	0.6588204077	0.044
Mitochondrial metabolism	Translocase of inner mitochondrial membrane 9 homolog (yeast)	TIMM9	0.6598542503	0.042
Cytokine signaling in immune system	B-cell CLL/lymphoma 6	BCL6	0.6760241074	0.0097
Cell cycle	Mutated in colorectal cancers	MCC	0.6761191711	0.0076
Gene expression	Zinc finger protein 519	ZNF519	0.6864230003	0.028
Gene expression	RNA binding motif protein, X-linked	RBMX	0.6983542235	0.012

In total, 6 genes were overexpressed greater than 1.4-fold in the nonalcoholic steatohepatitis (NASH) 70% PH group compared with their expression in the NASH 30% PH group, and 7 genes were downregulated by less than 0.70-fold in the NASH PH group compared with their expression in the NASH 30% PH group (n = 2). PH: Partial hepatectomy.