Proton pump inhibitor induced collagen expression in colonocytes is associated with collagenous colitis

Table 1 List of primer sets for RT-PCR

Gene	Symbol	Accession No.	Upper primer	Lower primer
Collagen type IV	Col4a1	NM_009931.2	AAAGGGAGAAAGAGGCTTGC	CCTTTGTACCGTTGCATCCT
Collagen type VI	Col6a1	NM_009933.4	GATGAGGGTGAAGTGGGAGA	CAGCACGAAGAGGATGTCAA
Collagen type I	Col1a1	NM_007742.4	GAGCGGAGAGTACTGGATCG	GCTTCTTTTCCTTGGGGTTC
Collagen type III	Col3a1	NM_009930.2	GCACAGCAGTCCAACGTAGA	TCTCCAAATGGGATCTCTGG
Solute carrier family 9, member A2	Slc9a2	NM_001033289.2	ACTGGGGTCACAACTTCTGG	CTTCACGGCAGTCATTGAGA
Transforming growth factor β1	Tgfb1	NM_011577.2	TTGCTTCAGCTCCACAGAGA	TGGTTGTAGAGGGCAAGGAC
Fibroblast growth factor 2	Fgf2	NM_008006.2	AGCGGCTCTACTGCAAGAAC	GCCGTCCATCTTCCTTCATA
Replication factor C1	Rfc1	NM_011258.2	TGATCACAGGAGTGCTGGAG	CGAGGATTTTTGTTCCCAGA
Glyceraldehyde-3-phosphate dehydrogenase	Gapdh	NM_001289726.1	AACTTTGGCATTGTGGAAGG	ACACATTGGGGGTAGGAACA

Table 2 Expression of collagen III and IV in collagenous colitis

Case no.	Age	Sex	PPI	Expression
				Collagen III	Collagen IV
1	79	F	+	++	++
2	71	F	+	+++	++
3	87	F	+	++	++
4	26	F	+	+++	++
5	80	M	+	+++	+
6	78	M	+	+++	++
7	80	F	-	++	+
8	45	M	-	+	++
9	67	M	-	++	+
10	59	F	-	+	++
11	77	F	-	++	++

PPI: Proton pump inhibitor.

Table 3 Expression of collagen type III and IV in proton pump inhibitor-asscoiated collagenous colitis

	PPI (+)	PPI (-)	P value
n	6	5
Age	70.2	65.6	NS
M:F	2:4	2:3	NS
Collagen
Type III	2.67 ± 0.52	1.6 ± 0.55	0.0089
Type IV	1.83 ± 0.41	1.6 ± 0.55	NS