Long-term culture-induced phenotypic difference and efficient cryopreservation of small intestinal organoids by treatment timing of Rho kinase inhibitor

Table 1 Primer sequences used in quantitative polymerase chain reaction analysis

Gene	Primer sequences (5’-3’)		Annealing temperatures (°C)
mLgr5	F	ACATTCCCAAGGGAGCGTTC	60
	R	ATGTGGTTGGCATCTAGGCG
mLyz1	F	GCCAAGGTCTACAATCGTTGTGAGTTG	60
	R	CAGTCAGCCAGCTTGACACCACG
mMuc2	F	ATGCCCACCTCCTCAAAGAC	60
	R	GTAGTTTCCGTTGGAACAGTGAA
mChgA	F	CCCACTGCAGCATCCAGTT	60
	R	AGTCCGACTGACCATCATCTTTC
mALP	F	AACTCACCTCATGGGCCTCTT	60
	R	GGGTTTCGGTTGGCATCATA
mGAPDH	F	TCATCAACGGGAAGCCCATCAC
	R	AGACTCCACGACATACTCAGCACCG

GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.