Mo XQ, Wei HY, Huang GR, Xu LY, Chen YL, Qi J, Xian W, Qin YC, Wei LD, Zhao LJ, Huang YQ, Xing W, Pu HQ, Wei PY, Li CG, Liang QC. Molecular mechanisms of apoptosis in hepatocellular carcinoma cells induced by ethanol extracts of Solanum lyratum Thumb through the mitochondrial pathway. World J Gastroenterol 2017; 23(6): 1010-1017 [PMID: 28246474 DOI: 10.3748/wjg.v23.i6.1010]
Corresponding Author of This Article
Dr. Yan-Qiang Huang, Professor, Breeding Base of Microbial Infection Key Laboratory in the West Guangxi, Youjiang Medical University for Nationalities, No. 98 Countryside Road, Baise 533000, Guangxi Zhuang Autonomous Region, China. hyq77615@163.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Table 2 Comparison of the inhibition rates of proliferation of hepatocellular carcinoma SMMC-7721 cells in the groups with different Solanum lyratum Thumb concentrations, -x ± s
Group
Concentration, mg/L
n
OD value
Inhibition rate, %
Three ST groups with different concentrations
2.5
12
1.516 ± 0.205
18.22 ± 2.32
5
12
1.242 ± 0.236
36.56 ± 2.51
10
12
0.815 ± 0.276
55.98 ± 3.12
Negative control group
0
12
1.842 ± 0.183
-
Positive control group
2.5
12
1.135 ± 0.172
38.60 ± 1.78
Table 3 Comparison of apoptosis rates of hepatocellular carcinoma SMMC-7721 cells in different groups, -x ± s
Group
Concentration, C/mg•L-1
Apoptosis rate of SMMC-7721 cells, %
Three ST groups with different concentrations
2.5
10.75 ± 2.51
5
17.31 ± 2.33
10
30.21 ± 3.62
Negative control group
0
3.75 ± 1.23
Positive control group
2.5
17.36 ± 1.62
Table 4 mRNA expressions of genes relating to hepatocellular carcinoma SMMC-7721 cells in each group
Group
Concentration, C/mg•L-1
mRNA expression of genes
Fas
FasL
Caspase-8
Caspase-3
p53
Bcl-2
β-actin
Three ST groups with different concentrations
2.5
52.47 ± 0.10
58.25 ± 0.51
30.57 ± 0.10
22.15 ± 0.02
34.17 ± 0.01
36.28 ± 0.14
17.53 ± 0.14
5
52.74 ± 0.08
56.81 ± 0.30
30.76 ± 0.03
22.33 ± 0.09
34.45 ± 0.20
35.40 ± 0.51
18.15 ± 0.41
10
53.41 ± 0.64
55.94 ± 0.80
31.30 ± 0.24
23.01 ± 0.47
35.11 ± 0.69
33.54 ± 0.51
19.10 ± 0.91
Negative control group
0
52.32 ± 0.04
59.13 ± 0.07
30.44 ± 0.06
22.05 ± 0.03
34.11 ± 0.02
37.59 ± 0.33
17.30 ± 0.17
Positive control group
2.5
52.79 ± 0.11
56.69 ± 0.34
30.77 ± 0.03
22.35 ± 0.12
34.43 ± 0.20
35.53 ± 0.35
18.18 ± 0.41
Table 5 Differences in mRNA expressions of genes relating to hepatocellular carcinoma SMMC-7721 cells in each group